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N5415

Sigma-Aldrich

Nerve Growth Factor-ß human

recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

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Synonym(s):
β-NGF, BNGF, NGF-β, NGF-beta
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤16 ng/mL ED50/EC50

mol wt

protein calculated mol wt 13.5 kDa (Monomer)

packaging

pkg of 10 μg
pkg of 100 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg Endotoxin (β-NGF)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... NGF(4803)

General description

NGF-β gene is mapped to human chromosome 1p13.2.
Recombinant human β-NGF is expressed in human 293 cells as a homodimer (non-disulfide bonded) of 120 amino acids, with a calculated molecular weight of 13.5 kDa (monomer). Nerve growth factor β (β-NGF) is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The production of natural β-NGF occurs in epithelial cells, and the pituitary gland.

Biochem/physiol Actions

A mutation in the NGF-β gene is implicated in the loss of pain perception.
Nerve growth factor β (β-NGF) is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

Caution

The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at -20 °C.

Physical form

This product is lyophilized from PBS.

Preparation Note

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile DDW containing 0.1% endotoxin-free recombinant human serum albumin, to a final concentration of 100 μg/ml.

Storage and Stability

Store product at –20 °C. The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at –20 °C.

Analysis Note

The specific activity is determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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