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N6009

Sigma-Aldrich

Nerve Growth Factor-2.5S from murine submaxillary gland

NGF-2.5S, lyophilized powder, suitable for cell culture

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Synonym(s):
NGF-2.5S
CAS Number:
MDL number:

biological source

mouse

Quality Level

form

lyophilized powder

potency

0.1-30 ng/mL EC50 (using PC-12 cells)

mol wt

26.5 kDa

packaging

pkg of 4X25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Ngf(18049)

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This Item
N0513N5415E1257
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

0.1-30 ng/mL EC50 (using PC-12 cells)

potency

2-250 ng/mL ED50/EC50

potency

≤16 ng/mL ED50/EC50

potency

0.02-2.0 ng/mL EC50

mol wt

26.5 kDa

mol wt

protein 130 kDa

mol wt

protein calculated mol wt 13.5 kDa (Monomer)

mol wt

-

packaging

pkg of 4X25 μg

packaging

pkg of 0.1 mg

packaging

pkg of 10 μg, pkg of 100 μg

packaging

pkg of 0.1 mg

storage condition

avoid repeated freeze/thaw cycles

storage condition

-

storage condition

-

storage condition

avoid repeated freeze/thaw cycles

General description

Nerve growth factor (NGF) is produced by different cell types: structural, accessory, and immune cells. It belongs to the neurotrophin family of trophic factors.

Application

Nerve Growth Factor-2.5S from murine submaxillary gland has been used:
  • in Ham′s F-12 medium for performing growth cone collapse assay using dorsal root ganglions (DRGs)
  • as a media supplement in the “process compartment” for microfluidic chamber studies
  • to treat mid-luteal cells to study its effect on choline acetyltransferase (CHAT) expression

Biochem/physiol Actions

Nerve growth factor (NGF) is neurotrophic and maintains sympathetic and sensory neurons of the peripheral nervous system and forebrain cholinergic neurons. NGF promotes the development of neurite-like filaments from chick embryo dorsal root ganglia and rat PC12 pheochromocytoma cells. NGF may have a role in in vivo fetal development and nerve regeneration especially, after ischemic, surgical, or chemical injuries. NGF may also be associated with the central nervous system. It exhibits ameliorating effects against ulcers (human cutaneous, corneal ulcers, and pressure ulcers), glaucoma, maculopathy, and retinitis pigmentosa. NGF-2.5S is a type of NGF-b originally derived under dissociative conditions and often varies from NGF-b due to proteolysis arising during its purification.

Other Notes

2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium acetate buffer.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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