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H9666

Sigma-Aldrich

Nerve Growth Factor β Human

NGF-β, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture

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Synonym(s):
NGF-β human
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤16 ng/mL ED50

quality

endotoxin tested

mol wt

13 kDa (non-glycosylated)

packaging

pkg of 10 μg

manufacturer/tradename

HumanZyme

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

storage temp.

−20°C

General description

HumanKine NGF-β is expressed in human 293 cells as a non-glycosylated, non-disulfide bonded 13 kDa homodimer.

NGF-β belongs to the nerve growth factor protein superfamily. It is a key growth factor in target-mediated regulation of peripheral innervation. During development, NGF-β regulates nerve cell death, synaptic connectivity, fiber guidance, and dendritic morphology. The presence of NGF-β in immune cells and endocrine cells as well as in the CNS limbic areas suggests that it may function as an intracellular messenger to regulate the body′s response to stress.
The nerve growth factor β (NGFB) gene is mapped to human chromosome 1p13.2.

Application

Nerve Growth Factor β Human has been used as a neurotrophic agent in luciferase reporter assay using mammalian cells.

Biochem/physiol Actions

Nerve Growth Factor β (NGFB) acts as a key mediator in neuronal and non-neuronal development, differentiation, and survival. Polymorphism of NGFB gene leads to hereditary sensory and autonomic neuropathies. Charcot arthropathy and thermal insensitivity are few of the related symptoms. NGFB is known to promote cancer cell proliferation and metastasis. It is associated with lung, breast, prostatic and pancreatic cancers. In animal models, NGFB is observed to prevent cholinergic neuron loss and enhances memory.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1X PBS

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

3D Insert is a trademark of 3D Biotek, LLC
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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