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N9532

Sigma-Aldrich

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

clone NOS-E1, ascites fluid

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Synonym(s):
Anti-eNOS
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NOS-E1, monoclonal

mol wt

antigen 135 kDa

contains

15 mM sodium azide

species reactivity

rat, mouse, human, bovine

technique(s)

microarray: suitable
western blot: 1:3,000 using whole cell extract of cultured bovine lung endothelial cells

isotype

IgA

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOS3(4846)
mouse ... Nos3(18127)
rat ... Nos3(24600)

Related Categories

General description

Monoclonal Anti-Nitric Oxide Synthase, endothelial (eNOS) (mouse IgA isotype) is derived from the NOS-E1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. NOS consists of heme and calmodulin binding sites located within its N-terminal half. It has been localized in many different cell types. Based on molecular mass, subcellular location and Ca2+ dependence, at least three types of NOS have been classified. Type I NOS (150-160 kD protein) is found in neurons and is also called as neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+-regulated NOS (cNOS). Type II (130 kD protein) is found in macrophages and is also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kD protein) is found in endothelial cells and is also called endothelial NOS (eNOS or ecNOS).
The gene encoding endothelial nitric oxide synthase (eNOS) is localized on chromosome 7q35-36 and has 26 exons.

Immunogen

synthetic peptide corresponding to nitric oxide synthase (NOS) from bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH. The immunogen sequence is highly conserved in human eNOS.

Application

Monoclonal Anti-Nitric Oxide Synthase, Endothelial (eNOS) has been used:
  • for enzyme linked immunosorbent assay (ELISA)
  • immunoblot
  • immunohistochemistry
  • immunofluorescence.

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse has been used in antibody microarray.

Biochem/physiol Actions

Endothelial nitric oxide synthase (eNOS) has been studied as a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated with coronary artery disease.
NOS catalyses the oxidation of guanidino nitrogen of arginine. The released nitric oxide mediates vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. NOS is associated with multiple sclerosis and allergic encephalomyelitis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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