P1435
Phosphatase, Acid from sweet potato
ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)
Synonym(s):
(Orthophosphoric-monoester phosphohydrolase [acid optimum] )
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About This Item
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form
ammonium sulfate suspension
Quality Level
specific activity
≥10.0 units/mg protein (modified Warburg-Christian)
secondary activity
≤9.9 units/mg protein ATPase
mol wt
110 kDa
storage temp.
2-8°C
SMILES string
O(CC(O)C)CC#C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
General description
Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points.
Application
Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase.
Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.
Biochem/physiol Actions
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO4 + H2O --------------- R-OH + HOPO3 2+Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.
Physical form
Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.3
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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D T Wyatt et al.
Clinical chemistry, 35(11), 2173-2178 (1989-11-01)
We analyzed extensively a modified thiochrome method for thiamin analysis. Acid phosphatase (EC 3.1.3.2) from potato was superior to either alpha-amylase or acid phosphatase from wheat germ as a dephosphorylating agent. Timing of cyanogen bromide exposure was important, but the
Mina Solhtalab et al.
Environmental science & technology, 56(22), 16441-16452 (2022-10-26)
Among ubiquitous phosphorus (P) reserves in environmental matrices are ribonucleic acid (RNA) and polyphosphate (polyP), which are, respectively, organic and inorganic P-containing biopolymers. Relevant to P recycling from these biopolymers, much remains unknown about the kinetics and mechanisms of different
Tatsuya Kusudo et al.
Bioscience, biotechnology, and biochemistry, 67(7), 1609-1611 (2003-08-13)
Purple acid phosphatase (PAP) was purified from sweet potato dry powder, which is used as a food additive. Spectrometric and enzymatic analyses, and analysis of the amino-terminal sequence indicated that the purified purple acid phosphatase was PAP1. High activity in
An unstable manganese(III) complex incorporating ligand donor types proposed for an acid phosphatase from sweet potato: (p-nitrobenzenethiolato)[N,N'-ethylenebis(salicylideneaminato)]manganese(III)
Gohdes, J. and W. Armstrong
Inorganic Chemistry, 27, 1841-1842 (1988)
H Kawabe et al.
Biochimica et biophysica acta, 784(1), 81-89 (1984-01-18)
The function of Mn(III) in plant acid phosphatase has been investigated by a metal-substitution study, and some properties of the Fe(III)-substituted enzyme were compared with those of the native Mn(III) enzyme and mammalian Fe(III)-containing acid phosphatases. 19F nuclear magnetic resonance
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Protocols
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
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