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P9156

Sigma-Aldrich

Plasminogen from bovine plasma

lyophilized powder, ≥2.0 units/mg protein

Synonym(s):

Profibrinolysin

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
42030240
NACRES:
NA.61

biological source

bovine plasma

Quality Level

form

lyophilized powder

specific activity

≥2.0 units/mg protein

concentration

2-7% protein (biuret)

suitability

not suitable for streptokinase determination by clot formation procedure

UniProt accession no.

application(s)

histology

storage temp.

2-8°C

Gene Information

cow ... PLG(280897)
human ... PLG(5340)

Biochem/physiol Actions

The activated form of plasminogen, plasmin, was shown to promote the migration of melanoma and lung tumor cells plated on the extracellular matrix protein laminin by altering protein kinase C signaling.
Plasminogen is the inactive precursor of the protease plasmin. Plasminogen is activated by the action of either tissue plasminogen activator (tPA), which primarily activates the fibrinolytic (thrombolytic) activity of plasmin, or urokinase plasminogen activator (uPA), which is associated with extracellular matrix remodeling and cell migration. Plasmin cleaves fibrin/fibrinogen and blood coagulation factors V/Va and VIII/VIIIa. It activates matrix metalloproteinases by cleaving the inactive proenzymes. It is also involved in the activation of some growth factors, such as vascular endothelial growth factor (VEGF) and transforming growth factor β (TGF-β).

Other Notes

ε-Aminocaproic acid free

Unit Definition

One unit will produce 1.0 μmole of p-nitroanilide from D-Val-Leu-Lys 4-nitroanilide at pH 7.5 at 37°C.

Physical form

Lyophilized powder containing NaCl, EDTA, lysine, and Tris buffer salts

Reconstitution

Solutions should be stored in buffer containing 20 mM lysine at neutral pH. The protein should be as concentrated as possible. Frozen in aliquots at −20 °C, it should be stable for several weeks.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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L Chikahisa et al.
Invasion & metastasis, 17(6), 323-333 (1997-01-01)
Using a pure chemotactic model, we investigated the effect of plasmin on tumor cell motility. In the presence of various extracellular matrix proteins, plasmin facilitated motility of human melanoma LOX and lung cancer Lu-99 cells. Laminin contributed most to the
Yueling Zhang et al.
FEBS letters, 587(9), 1304-1309 (2013-03-12)
Cluster 1 streptokinases (SK1) from Streptococcus pyogenes (GAS) show substantially higher human plasminogen (hPg) activation activities and tighter hPg binding affinities than cluster 2b streptokinases (SK2b) in solution. The extent to which the different domains of SK are responsible for
Barbara Ruszkowska et al.
Acta obstetricia et gynecologica Scandinavica, 92(7), 841-846 (2013-03-12)
To assess the concentration of adiponectin, soluble E-selectin, soluble thrombomodulin and tissue activator plasminogen antigen in postmenopausal women who received oral or transdermal hormone therapy. Case-control study. Polish university hospitals. Seventy-six healthy postmenopausal women. Forty-six women who received oral (n
Liver-artery interactions via the plasminogen-CD36 axis in macrophage foam cell formation: new evidence for the role of remote organ crosstalk in atherosclerosis.
Hiroshi Iwata et al.
Circulation, 127(11), 1173-1176 (2013-03-20)
Katie M Weigandt et al.
Biophysical journal, 103(11), 2399-2407 (2013-01-04)
Using a combination of structural and mechanical characterization, we examine the effect of fibrinogen oxidation on the formation of fibrin clots. We find that treatment with hypochlorous acid preferentially oxidizes specific methionine residues on the α, β, and γ chains

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