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RAB0182

Sigma-Aldrich

Human bFGF ELISA Kit

for serum, plasma, cell culture supernatant and urine

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Synonym(s):
Basic fibroblast growth factor, FGF-2, Fibroblast growth factor 2, HBGF-2, Heparin-binding growth factor 2, bFGF
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 102.4-10000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FGF2(2247)

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RAB0182

Human bFGF ELISA Kit

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Human HGF ELISA Kit

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 102.4-10000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 102.4-10000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.74-2000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.74-2000 pg/mL

Gene Information

human ... FGF2(2247)

Gene Information

human ... FGF2(2247)

Gene Information

human ... HGF(3082)

Gene Information

human ... HGF(3082)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The Human bFGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human bFGF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human basic-FGF

Application

Human bFGF ELISA kit has been used:
  • to quantify the level of residual cytokines in decellularized meningeal scaffolds (DMS)
  • to measure cytokine levels in acellularized meningeal scaffolds (AMS) and recovered meningeal tissues (RMS/CMS)
  • to analyze basic-fibroblast growth factor (bFGF) concentration in cell culture medium

Biochem/physiol Actions

FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3. It stimulates the proliferation of a wide variety of cells, including mesenchymal, neuroectodermal and endothelial cells. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair. FGF2 is known to stimulate angiogenesis and is associated with cancer development. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. MicroRNA (miR)-503 targets FGF2 mRNA and inhibits migration and invasiveness of osteosarcoma (OS) cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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FGF2 plays a key role in embryonic cerebrospinal fluid trophic properties over chick embryo neuroepithelial stem cells.
Martin C
Developmental Biology, 297(2), 402-416 (2006)
In Kyong Shim et al.
Journal of biomedical materials research. Part A, 102(10), 3639-3648 (2013-11-23)
Efficient osseointegration is a key factor in dental implants to reduce the total time-course of therapy. Titanium implants with anodized surface gained much interest for their enhanced osseointegration. Anodized implant combined with bioactive drugs is an ideal candidate for enhance
Yang Hu et al.
Molecular medicine reports, 13(1), 49-58 (2015-11-18)
Recent studies regarding regenerative medicine have focused on bone marrow mesenchymal stem cells (BMSCs), which have the potential to undergo neural differentiation, and may be transfected with specific genes. BMSCs can differentiate into neuron‑like cells in certain neurotropic circumstances in
Xiaohua Liu et al.
Psychiatry research, 218(1-2), 54-60 (2014-05-06)
Multiple neurotrophic and/or growth factors, recently nominated as "angioneurins", play the key roles in mood modulation and neuroplasticity, and their dysfunction might be involved in the pathophysiology and treatment of mood disorders. We examined serum levels of vascular endothelial growth
Sandeep Kumar Vishwakarma et al.
Materials science & engineering. C, Materials for biological applications, 102, 34-44 (2019-05-31)
Spinal cord injury (SCI) is one of the most precarious conditions which have been one of the major reasons for continuous increasing mortality rate of SCI patients. Currently, there is no effective treatment modality for SCI patients posing major threat

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