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RAB0197

Sigma-Aldrich

Human Ferritin ELISA Kit

for serum, plasma, cell culture supernatant and urine

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Synonym(s):
FTH, Ferritin H subunit, Ferritin heavy chain
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.81-200 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FTH1(2495)

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species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.81-200 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 500 pg/mL
standard curve range: 0.512-125 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 ng/mL
standard curve range: 1.10-800 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 250 pg/mL
standard curve range: 0.41-100 ng/mL

input

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

General description

The Human Ferritin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ferritin in serum, plasma, cell culture supernatants and urine.
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).

Immunogen

Recombinant Human Ferritin

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Ferritin ELISA Kit has been used to estimate the amount of ferritin in cellular suspension.
Human ferritin ELISA kit. has been used to measure the ferritin secreted in supernatants from THP-1 (tamm-horsfall protein 1) cells that were sequentially treated with lipopolysaccharides (LPS) and LLOMe (a lysosome disruption agent).

Biochem/physiol Actions

Ferritin mediates iron metabolism and plays a key role in redox homeostasis. Ferritin possesses ferroxidase activity and maintains iron in its non-toxic form. Ferritin acts as an iron-storage protein and stores excessive intracellular iron that is not utilized for the production of heme. Also, iron is released from ferritin upon degradation.
Ferritin/ ferritin heavy chain 1 (FTH1) is a cytoplasmic protein. It acts as a cargo for transport to autophagosomes for degradation. FTH1 maintains the cellular redox status by storing the intracellular iron in a non-toxic and readily available form. It acts as a key antioxidant molecule in various physiologic and pathologic conditions, such as apoptosis, inflammation, vascular protection and neurodegeneration. FTH1 alone or along with ferritin light chain is implicated to detect cancer cells, stem cells, fibroblasts and dendritic cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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