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RAB0386

Sigma-Aldrich

Brain Natriuretic Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

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Synonym(s):
Gamma-brain natriuretic peptide, Natriuretic peptides B , BNP
NACRES:
NA.32

species reactivity

human, mouse, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type serum
sample type culture supernatant(s)
sample type plasma
sample type cell lysate

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.66 pg/mL
standard curve range: 0.1–1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NPPB(4879)

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RAB0386

Brain Natriuretic Peptide EIA Kit

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RAB0413

Peptide YY EIA Kit

species reactivity

human, mouse, rat

species reactivity

mouse, human, rat

species reactivity

rat, mouse, human

species reactivity

mouse, human, rat

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

Gene Information

human ... NPPB(4879)

Gene Information

human ... NPPA(4878)

Gene Information

human ... PNPLA3(80339)

Gene Information

human ... PYY(5697)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.66 pg/mL
standard curve range: 0.1–1000 pg/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 21 pg/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 5.6 pg/mL
standard curve range: 3.91-1000 pg/mL

input

sample type serum
sample type culture supernatant(s)
sample type plasma
sample type cell lysate

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)

input

sample type cell lysate
sample type plasma
sample type culture supernatant(s)
sample type serum

input

sample type serum
sample type cell lysate
sample type culture supernatant(s)

General description

The BNP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting BNP peptide based on the principle of Competitive Enzyme Immunoassay.

Immunogen

Brain Natriuretic Peptide, synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Brain natriuretic peptide EIA Kit has been used to measure brain natriuretic peptide (BNP) secreted in human neonatal ventricular cardiomyocyte culture.

Biochem/physiol Actions

Brain natriuretic peptide (BNP) expressed in mammalian brain and atrial natriuretic peptide (ANP) facilitates various physiological functions including regulation of blood pressure and cardiac remodeling. BNP acts as a potential biomarker of high left ventricular end-diastolic pressure in patients with symptomatic left ventricular (LV) dysfunction. Elevated expression of BNP is observed in acute myocardial infarction (AMI) patients. BNP is also implicated in several biological functions such as diuresis, natriureis, hypotensive action and inhibition of aldosterone secretion.

Kit Components Also Available Separately

Product No.
Description
SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Plasma brain natriuretic peptide as a biochemical marker of high left ventricular end-diastolic pressure in patients with symptomatic left ventricular dysfunction.
Maeda K
American Heart Journal, 135(5 pt 1), 825-832 (1998)
Azar Hosseini et al.
Frontiers in pharmacology, 13, 909079-909079 (2022-06-28)
Background: Doxorubicin as an anti-cancer drug causes cardiotoxicity, limiting its tolerability and use. The mechanism of toxicity is due to free radical production and cardiomyocytes injury. This research evaluated Rheum turkestanicum (R.turkestanicum) extract against doxorubicin cardiotoxicity due to its considerable
A new natriuretic peptide in porcine brain.
Sudoh T
Nature, 332(6159), 78-81 (1988)
Induction of HO-1 by carbon monoxide releasing molecule-2 attenuates thrombin-induced COX-2 expression and hypertrophy in primary human cardiomyocytes.
Chien PT
Toxicology and Applied Pharmacology, 289(2), 349-359 (2015)
Distinct T cell signatures define subsets of patients with multiple sclerosis.
Johnson MC
Neurology? neuroimmunology & neuroinflammation, 3(5), e278-e278 (2016)

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