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RAB0385

Sigma-Aldrich

Atrial Natriuretic Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

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Synonym(s):
Natriuretic peptides A, Prepronatriodilatin, ANP
NACRES:
NA.32

species reactivity

mouse, human, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NPPA(4878)

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detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

Gene Information

human ... NPPA(4878)

Gene Information

human ... NPPB(4879)

Gene Information

human ... PNPLA3(80339)

Gene Information

human ... PYY(5697)

species reactivity

mouse, human, rat

species reactivity

human, mouse, rat

species reactivity

rat, mouse, human

species reactivity

mouse, human, rat

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)

input

sample type serum
sample type culture supernatant(s)
sample type plasma
sample type cell lysate

input

sample type cell lysate
sample type plasma
sample type culture supernatant(s)
sample type serum

input

sample type serum
sample type cell lysate
sample type culture supernatant(s)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.66 pg/mL
standard curve range: 0.1–1000 pg/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 21 pg/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 5.6 pg/mL
standard curve range: 3.91-1000 pg/mL

General description

The ANP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting ANP peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANP-1 targets the common sequence of human, mouse and rat, and thus may be used to detect ANP expression in all these species with high specificity and sensitivity.

Immunogen

Atrial Natriuretic Peptide, synthetic peptide

Application

Atrial Natriuretic Peptide EIA Kit has been used for the quantification of atrial natriuretic peptide (ANP) in cell culture.
For research use only. Not for use in diagnostic procedures.
Atrial Natriuretic Peptide EIA Kit has been used to measure ANP levels in diabetes mellitus patients.

Biochem/physiol Actions

Atrial natriuretic peptide (ANP) is a polypeptide hormone released in response to atrial dilation and it stimulates diuresis, natriuresis and vasorelaxation. ANP acts as an inhibitor of renin-angiotensin-aldosterone system (RAAS) pathway. It also has a major role in body fluid homeostasis and maintenance of blood pressure. Binding of ANP to receptors on target cell stimulate the production of cyclic guanosine monophosphate (GMP). Overexpression of ANP leads to congestive heart failure in the human. Experimental studies show that ANP can be used as a therapeutic for stimulating angiogenesis in ischemic tissues. N-terminal ANP prohormone functions as a biomarker for asymptomatic systolic left ventricular (LV) dysfunction, congestive heart failure and cardiovascular mortality.

Kit Components Also Available Separately

Product No.
Description
SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Customers Also Viewed

Natriuretic peptides: Diagnostic and therapeutic use.
Pandit K
Indian Journal of Endocrinology and Metabolism, suppl 4, S345-S353 (2011)
Atrial natriuretic peptide elevation in congestive heart failure in the human.
Burnett JC Jr
Science, 231(4742), 1145-1147 (1986)
Joseph A Hippensteel et al.
Critical care (London, England), 23(1), 259-259 (2019-07-25)
Intravenous fluids, an essential component of sepsis resuscitation, may paradoxically worsen outcomes by exacerbating endothelial injury. Preclinical models suggest that fluid resuscitation degrades the endothelial glycocalyx, a heparan sulfate-enriched structure necessary for vascular homeostasis. We hypothesized that endothelial glycocalyx degradation
Endothelial cell modulation of cardiomyocyte gene expression
Jiang F, et al.
Experimental Cell Research, 383(2), 111565-111565 (2019)
Brain natriuretic peptide as a novel cardiac hormone in humans. Evidence for an exquisite dual natriuretic peptide system, atrial natriuretic peptide and brain natriuretic peptide.
Mukoyama M
The Journal of Clinical Investigation, 87(4), 1402-1412 (1991)

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