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RAB0747

Sigma-Aldrich

Human TF / Serotransferrin ELISA Kit

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Synonym(s):
Serotransferrin, TF, Transferrin
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 ng/mL
standard curve range: 1.029-750 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TF(7018)

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input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type urine
sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 ng/mL
standard curve range: 1.029-750 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.81-200 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 6.14-1500 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 220 pg/mL
standard curve range: 0.205-50 ng/mL

Gene Information

human ... TF(7018)

Gene Information

human ... FTH1(2495)

Gene Information

human ... F3(2152)

Gene Information

human ... TG(7038)

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

General description

Transferrin (TF) is an iron (Fe) transport protein and found in blood plasma and brain extracellular fluids. It belongs to the family of Fe-binding glycoproteins. TF is made of a single polypeptide chain with two domains and each domain consists of a Fe-binding site. It helps in the transport of Fe(III) between the cells in soluble and non-toxic forms. TF also acts as a bacteriostatic agent as well as a growth factor. It binds to the transferrin receptor to mediate the cellular uptake of bound Fe. The TF gene is mapped on the human chromosome at 3q22.1. The antibody pair provided in this kit recognizes human serotransferrin.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Trace elements, like Cu, Zn, Fe, or Se, are important for the proper functioning of antioxidant enzymes. However, in excessive amounts, they can also act as pro-oxidants. Accordingly, trace elements influence redox-modulated signaling pathways, such as the Nrf2 pathway. Vice
Akira Wada et al.
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Stefanie Dichtl et al.
mBio, 10(1) (2019-02-07)
We have recently shown that the catecholamine dopamine regulates cellular iron homeostasis in macrophages. As iron is an essential nutrient for microbes, and intracellular iron availability affects the growth of intracellular bacteria, we studied whether dopamine administration impacts the course

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