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S5897

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, fine

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CAS Number:
MDL number:
NACRES:
NA.52

grade

DNA grade
for molecular biology

product line

BioReagent

form

beads

swelling

1 g swells to 9-11 mL

bead diameter

20-80 μm

pore size

exclusion limit (20 bp dsDNA (rA)20)

pH

2-10

foreign activity

DNase, RNase, none detected

storage temp.

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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1 of 4

This Item
S6022G5050GE17-0042-02
Sephadex® G-50 BioReagent, for molecular biology, DNA grade, fine

Sigma-Aldrich

S5897

Sephadex® G-50

Sephadex® G-50 BioReagent, for molecular biology, DNA grade, medium

Sigma-Aldrich

S6022

Sephadex® G-50

Sephadex® G-50 Superfine

Sigma-Aldrich

G5050

Sephadex® G-50

Sephadex® G-50 Fine, Cytiva, pack of 500 g

GE17-0042-02

Sephadex® G-50

product line

BioReagent

product line

BioReagent

product line

-

product line

-

form

beads

form

beads

form

-

form

-

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

swelling

1 g swells to 9-11 mL

swelling

1 g swells to 9-11 mL

swelling

1 g swells to 9-11 mL

swelling

1 g swells to 9-11 mL

General description

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Application

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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1000309185

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The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve

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