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S6147

Sigma-Aldrich

Sephadex® G-100

BioReagent, for molecular biology, DNA grade, medium

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CAS Number:
MDL number:
NACRES:
NA.52

grade

DNA grade
for molecular biology

Quality Level

product line

BioReagent

form

beads

swelling

1 g swells to 9-11 mL

bead diameter

40-120 μm

pore size

exclusion limit (25 bp dsDNA; (rA)27)

pH

2-10

foreign activity

DNase, RNase, none detected

storage temp.

room temp

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This Item
G10120G10050G100120
Sephadex® G-100 BioReagent, for molecular biology, DNA grade, medium

Sigma-Aldrich

S6147

Sephadex® G-100

Sephadex® G-10 Medium

Sigma-Aldrich

G10120

Sephadex® G-10

Sephadex® G-100 Superfine

Sigma-Aldrich

G10050

Sephadex® G-100

Sephadex® G-100 Medium

Sigma-Aldrich

G100120

Sephadex® G-100

product line

BioReagent

product line

-

product line

-

product line

-

form

beads

form

-

form

-

form

slurry

swelling

1 g swells to 9-11 mL

swelling

1 g swells to 2-3 mL

swelling

1 g swells to 15-20 mL

swelling

1 g swells to 15-20 mL gel

bead diameter

40-120 μm

bead diameter

-

bead diameter

-

bead diameter

-

pore size

exclusion limit (25 bp dsDNA; (rA)27)

pore size

-

pore size

-

pore size

-

General description

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
Sanjiv K Mishra et al.
Protein expression and purification, 80(2), 234-238 (2011-07-13)
C-phycoerythrin was isolated and purified from marine Pseudanabaena sp. using two step chromatographic methods. Phycobiliproteins in the marine Pseudanabaena was extracted in 100 mM phosphate buffer (pH 7.2) and precipitated by salting out. The precipitated C-phycoerythrin was purified by gel
Fernanda O Lemos et al.
European journal of dermatology : EJD, 21(5), 722-730 (2011-07-09)
Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of
Yen-Hui Lin
Water science and technology : a journal of the International Association on Water Pollution Research, 66(12), 2604-2612 (2012-10-31)
Molecular weight (MW) distribution of organic matter by ozonation and biofiltration was evaluated using gel chromatography. The MW distribution of organic matter by Sephadex G-25 was observed from groups 2 (MW = 1,029-7,031 g/mol) and 3 (MW = 303-1,029 g/mol)

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