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S7901

Sigma-Aldrich

Stem Cell Factor human

SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

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Synonym(s):
SCF, c-Kit ligand
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

powder

potency

1-5 ng/mL ED50

quality

endotoxin tested

mol wt

protein 18.5 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... KITLG(4254)

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Application

Stem Cell Factor human has been used in
  • In vitro differentiation of equine embryonic stem cells
  • Chemotaxis detection system
  • Ex vivo expansion of umbilical cord blood CD34+ cells
  • Cytokine therapy

Biochem/physiol Actions

Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine. SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS containing 50 μg BSA per 1 μg as a carrier protein.

Analysis Note

The proliferative activity is tested in culture using TF1 human erythroleukemic cells.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Roberto A Rovasio et al.
European journal of cell biology, 91(5), 375-390 (2012-03-03)
The extracellular environment through which neural crest cells (NCCs) translocate and differentiate plays a crucial role in the determination of cell migration and homing. In the trunk, NCC-derived melanocyte precursor cells (MPCs) take the dorsolateral pathway and colonize the skin
Isolation and ex vivo expansion of human umbilical cord blood-derived CD34+ stem cells and their cotransplantation with or without mesenchymal stem cells.
Delalat, B, et al.
Hematology, 14.3, 375-390 (2009)
Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells
Saito S, et al.
Methods in Molecular Biology, 329, 59-79 (2006)
Insights into Stem Cell Factor chemotactic guidance of neural crest cells revealed by a real-time directionality-based assay
Rovasio RA, et al.
European Journal of Cell Biology, 91, 375-390 (2012)
L K Ashman
The international journal of biochemistry & cell biology, 31(10), 1037-1051 (1999-12-03)
The receptor tyrosine kinase c-Kit and its ligand Stem Cell Factor (SCF) are essential for haemopoiesis, melanogenesis and fertility. SCF acts at multiple levels of the haemopoietic hierarchy to promote cell survival, proliferation, differentiation, adhesion and functional activation. It is

Articles

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