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SAB1305538

Sigma-Aldrich

MONOCLONAL ANTI-HIS TAG antibody produced in mouse

clone 6AT18, IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Mouse Anti-Hexa Histidine tag
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

6AT18, monoclonal

form

buffered aqueous solution

technique(s)

western blot: 1:1000

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
A5588H1029A5713
antibody form

IgG fraction of antiserum

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

6AT18, monoclonal

clone

HIS-1, monoclonal

clone

HIS-1, monoclonal

clone

HIS-1, monoclonal

technique(s)

western blot: 1:1000

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

technique(s)

dot blot: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

technique(s)

-

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

UniProt accession no.

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

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General description

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent′s anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

Immunogen

Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.

Physical form

Supplied in PBS with 0.09% (W/V) sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jialing Bao et al.
Frontiers in cellular and infection microbiology, 11, 694957-694957 (2021-06-08)
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Methods in molecular biology (Clifton, N.J.), 1813, 125-148 (2018-08-12)
The amoeba Dictyostelium discoideum is a single-cell organism that can undergo a simple developmental program, making it an excellent model to study the molecular mechanisms of cell motility, signal transduction, and cell-type differentiation. A variety of human genes that are
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It is widely believed that inflammation associated with obesity has an important role in the development of type 2 diabetes. IκB kinase beta (IKKβ) is a crucial kinase that responds to inflammatory stimuli such as tumor necrosis factor α (TNF-α)

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