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11965085001

Roche

Anti-His6-Peroxidase

from mouse IgG1

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Synonym(s):
antibody

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BMG-his-1, monoclonal

form

lyophilized

packaging

pkg of 50 U

manufacturer/tradename

Roche

isotype

IgG1

storage temp.

2-8°C

General description

Anti-His6-Peroxidase is a monoclonal antibody to His6-tagged proteins, conjugated to horseradish peroxidase. Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the epitope-sequence location; however, it preferentially recognizes the C-terminal His6 epitope with high sensitivity.

Specificity

Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the epitopesequence. However, Anti-His6-Peroxidase preferentially recognizes the C-terminal His6 epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.

Application

Anti-His6-Peroxidase has been used in in vitro serum stability of fusion toxins, pull-down assay and western blotting.
Anti-His6-Peroxidase is used for the detection of His6-tagged proteins in:
  • ELISA (enzyme-linked immunosorbent assay)
  • Western blot

Quality

Function test: Western blot using extracts from cell line expressing a recombinant His6-tagged protein.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.


The following concentrations should be taken as a guideline:
  • ELISA: 100 mU/ml
  • Western blot: 100mU/ml

Reconstitution

Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Reconstitution should be performed for at least 10 minutes.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash


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