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71104-M

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Sf9 Insect Cells - Novagen

Serum-free, adapted Sf9 cells

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Synonym(s):
InsectDirect™ System- Sf9 Cells

biological source

(Spodoptera frugiperda (Sf9))

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze

growth mode

adherent or suspension

morphology

epithelial

technique(s)

microbiological culture: suitable

shipped in

dry ice

storage temp.

-140 to -196°C

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Sf9 Insect Cells - Novagen Serum-free, adapted Sf9 cells

Sigma-Aldrich

71104-M

Sf9 Insect Cells - Novagen

Insect GeneJuice® Transfection Reagent Proprietary liposome transfection reagent optimized for maximal transfection efficiency of Sf9 insect cells for baculovirus protein expression.

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Insect GeneJuice® Transfection Reagent

pIEx-4 DNA - Novagen Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

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pIEx-4 DNA - Novagen

pIEx-5 DNA - Novagen® Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

Sigma-Aldrich

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pIEx-5 DNA - Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

growth mode

adherent or suspension

growth mode

-

growth mode

-

growth mode

-

morphology

epithelial

morphology

-

morphology

-

morphology

-

technique(s)

microbiological culture: suitable

technique(s)

transfection: suitable

technique(s)

-

technique(s)

-

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Sf9 Insect Cells are provided as frozen stocks of Spodoptera frugiperda Sf9 cells for establishment of cultures suitable for any application. These cells plus BacVector Insect Cell Medium are recommended for cotransfection of transfer plasmids with BacVector Triple Cut Virus DNA or BacMagicDNA for the construction of baculovirus recombinants and for transfection of pIEx and pBiEx vector constructs of the InsectDirect System. After recovery, they can be grown as semi-adherent cultures in tissue culture flasks or in suspension as shaker cultures. Cells are shipped on dry ice. Upon receipt, the vials should be removed from their container and either recovered immediately, or placed at -70°C and used within two weeks. For longer-term storage, place in liquid nitrogen.



This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Components

•: 3 vials: Sf9 Insect Cells (frozen at 1 x 10⁷ cells/ml)

Warning

Toxicity: Irritant (B)

Legal Information

INSECTDIRECT is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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