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T1032

Sigma-Aldrich

TNM-FH insect medium

With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture

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Synonym(s):
Graces Modified Medium
MDL number:
NACRES:
NA.75

Quality Level

form

powder

concentration

50.6 g/L

technique(s)

cell culture | insect: suitable

suitability

suitable for culture of other lepidopteran cell lines (Originally developed for the culture of Trichoplusia ni cells)

components

L-glutamine: 0.6 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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This Item
51942CT3285G8142
Sigma-Aldrich

Sigma-Aldrich

T1032

TNM-FH insect medium

SAFC

SAFC

51942C

Hink′s TNM-FH Insect Medium

Sigma-Aldrich

Sigma-Aldrich

T3285

TNM-FH insect medium

concentration

50.6 g/L

concentration

-

concentration

-

concentration

-

technique(s)

cell culture | insect: suitable

technique(s)

cell culture | insect: suitable

technique(s)

cell culture | insect: suitable

technique(s)

cell culture | insect: suitable

suitability

suitable for culture of other lepidopteran cell lines (Originally developed for the culture of Trichoplusia ni cells)

suitability

-

suitability

-

suitability

-

components

L-glutamine: 0.6 g/L
NaHCO3: no

components

L-glutamine: 4.1 mM

components

NaHCO3: 0.35 g/L
L-glutamine: 0.6 g/L

components

NaHCO3: 0.35 g/L
L-glutamine: 0.6 g/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

A number of modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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To facilitate studies of the development and membrane biophysics of cells in the central olfactory pathway of the moth Manduca sexta, we have dissociated neurons and glial cells from the antennal lobes (ALs) and cultivated them in the controlled environment
H Inohara et al.
Cancer research, 55(15), 3267-3271 (1995-08-01)
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W J Shen et al.
The Journal of biological chemistry, 276(52), 49443-49448 (2001-10-30)
Hormone-sensitive lipase (HSL) is an intracellular lipase that plays an important role in the hydrolysis of triacylglycerol in adipose tissue. HSL has been shown to interact with adipocyte lipid-binding protein (ALBP), a member of the family of intracellular lipid-binding proteins
S E Crawford et al.
Journal of virology, 68(9), 5945-5952 (1994-09-01)
Rotaviruses are triple-layered particles that contain four major capsid proteins, VP2, VP4, VP6, and VP7, and two minor proteins, VP1 and VP3. We have cloned each of the rotavirus genes coding for a major capsid protein into the baculovirus expression

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