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T3285

Sigma-Aldrich

TNM-FH insect medium

With ʟ-glutamine and sodium bicarbonate,liquid, sterile-filtered, suitable for insect cell culture

Synonym(s):

supplemented Grace′s medium

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About This Item

MDL number:
MFCD00243216
UNSPSC Code:
12352207
NACRES:
NA.75

Product Name

TNM-FH insect medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | insect: suitable

components

NaHCO3: 0.35 g/L
L-glutamine: 0.6 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

Several modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species. 

Application

TNM-FH insect medium has been used to culture Sf9 insect cells.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000403182
0000403183
0000403182
0000403183
0000309304

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Thomas Piechulek et al.
The Journal of biological chemistry, 280(47), 38923-38931 (2005-09-21)
The regulation of the two isoforms of phospholipase C-gamma, PLCgamma(1) and PLCgamma(2), by cell surface receptors involves protein tyrosine phosphorylation as well as interaction with adapter proteins and phosphatidylinositol 3,4,5-trisphosphate (PtdInsP(3)) generated by inositol phospholipid 3-kinases (PI3Ks). All three processes
J J Gallardo-Rodríguez et al.
Bioresource technology, 200, 699-705 (2015-11-12)
A modification of a flow contraction device was used to subject shear-sensitive microalgae to well-defined hydrodynamic forces. The aim of the study was to elucidate if the inhibition of shear-induced growth commonly observed in dinoflagellate microalgae is in effect due
Effects of media constituents on growth of insect cells in stationary and suspension cultures.
Hink, W.F., et al.
In Vitro, 9, 371-371 (1974)
Established insect cell line from the cabbage looper, Trichoplusia ni.
W F Hink
Nature, 226(5244), 466-467 (1970-05-02)
Boglárka Zámbó et al.
Scientific reports, 8(1), 7487-7487 (2018-05-12)
The ABCG2 membrane protein is a key xeno- and endobiotic transporter, modulating the absorption and metabolism of pharmacological agents and causing multidrug resistance in cancer. ABCG2 is also involved in uric acid elimination and its impaired function is causative in
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