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T3160

Sigma-Aldrich

TC-100 Insect Medium

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

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Synonym(s):
BML-TC/10
MDL number:
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | insect: suitable

components

glucose: 1.0 g/L
L-glutamine: 0.6 g/L
NaHCO3: 0.35 g/L

shipped in

ambient

storage temp.

2-8°C

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I7760T103214380C
Sigma-Aldrich

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T3160

TC-100 Insect Medium

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I7760

IPL-41 Insect Medium

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T1032

TNM-FH insect medium

form

liquid

form

liquid

form

powder

form

liquid

technique(s)

cell culture | insect: suitable

technique(s)

cell culture | insect: suitable

technique(s)

cell culture | insect: suitable

technique(s)

cell culture | insect: suitable

components

glucose: 1.0 g/L
L-glutamine: 0.6 g/L
NaHCO3: 0.35 g/L

components

NaHCO3: 0.25 g/L

components

L-glutamine: 0.6 g/L
NaHCO3: no

components

NaHCO3: no
L-glutamine: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

TC-100 Insect Medium has been developed to optimize the production of Autographica californica nuclear polyhedrosis virus (NPV) virions by cells from the fall armyworm, Spodoptera frugiperda. It has been found to support the growth of cells derived from a variety of lepidopteran species. The medium is mostly employed in the growth of baculoviruses in lepidopteran cell lines.

Application

For baculovirus culturing in lepidoptera.
TC-100 Insect Medium has been used:
  • in the transfection of insect cells in suspension for efficient baculovirus generation
  • to harvest Varroa destructor saliva
  • as a starter media to culture and maintain insect cells such as Spodoptera frugiperda

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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MethodsX, 3, 371-377 (2016-05-26)
Baculovirus (BV) mediated insect cell expression system utilizes transfection as a first step to introduce recombinant baculovirus DNA into insect cells. Many labs are still relying on the conventional liposome based transfection method in adherent culture. Here we describe a
Insect Biotechnology
Short Views on Insect Genomics and Proteomics, 185-210 (2016)
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Journal of economic entomology, 112(2), 505-514 (2018-09-17)
The Varroa destructor (Acari Varroidae) mite is a serious threat to honey bee due to hemolymph feeding and virus transmission. Mite salivary proteins are involved in these interactions. However, the salivary secretome has not been previously characterized. In this paper

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