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I7760

Sigma-Aldrich

IPL-41 Insect Medium

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Insect Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

IPL-41 Insect Medium, With L-glutamine and sodium bicarbonate., liquid, sterile-filtered, suitable for insect cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | insect: suitable

components

NaHCO3: 0.25 g/L

shipped in

ambient

storage temp.

2-8°C

Application

IPL-41 Insect Medium has been used in culturing silk worm cell lines.
IPL-41 is part of a series of media originally developed at the USDA Insect Pathology Laboratory by Weiss et al. for the large scale propagation of cells from the fall armyworm, Spodoptera frugiperda. The medium is primarily used for the growth and maintenance of cell lines derived from lepidopterans and the propagation of viruses in these cell lines. One of the most significant uses of the medium is the large scale culture of baculovirus infected Spodoptera cells. The expression of recombinant proteins is undertaken from Spodoptera cells utilizing the baculovirus expression system (BEVS). Attempts have also been made to obtain the expression of foreign genes in cells cultured in IPL-41 without serum supplementation.

pictograms

CorrosionExclamation mark
GHS05,GHS07

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Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Masashi Iwanaga et al.
Journal of invertebrate pathology, 101(2), 124-129 (2009-05-23)
A new cell line, designated as NIAS-Boma-529b, was established from the larval fat bodies of Bombyx mandarina (B. mandarina), which is believed to be an ancestor of Bombyx mori (B. mori). This cell line has been cultured for approximately 150
Chromatin-induced spindle assembly plays an important role in metaphase congression of silkworm holocentric chromosomes
Mon H, et al.
Insect Biochemistry and Molecular Biology, 45(4), 40-50 (2014)
Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1
Mon H, et al.
RNA Biology, 9(1), 40-46 (2012)
Proteasome inhibitor MG132 impairs autophagic flux through compromising formation of autophagosomes in Bombyx cells
Ji M M, et al.
Biochemical and biophysical research communications, 479(4), 690-696 (2016)
Ming-Ming Ji et al.
Biochemical and biophysical research communications, 479(4), 690-696 (2016-10-04)
MG132 has been used as a proteasome inhibitor on Bombyx cells, but its physiological effects on autophagy still have not been elucidated. In this study, we find that the lipidated BmAtg8, BmAtg8-PE as an autophagosomal marker protein, is only localized
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