11922416001

Anti-His₆

from mouse IgG₁

• Synonym(s): metal chelate affinity chromatography
• UNSPSC Code: 12352203
• NACRES: NA.54
• Conjugate: unconjugated
• Clone: monoclonal
• Application: —
• Citations: 17

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: monoclonal
• form: lyophilized
• packaging: pkg of 100 μg
• manufacturer/tradename: Roche
• isotype: IgG1
• storage temp.: 2-8°C

Description

General description

Anti-His₆ is a monoclonal antibody to His₆-tagged proteins. Anti-His₆ specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence.

Application

Anti-His₆ is used for the detection of His₆-tagged proteins in:

• Immunoblotting (e.g., dot blots and western blots)
• Immunoprecipitation
• Immunoassays (ELISA)
• Immunocytochemistry
• Immunofluorescence

Biochem/physiol Actions

Anti-His₆ specifically recognizes an epitope of six consecutive histidine residues (His₆) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the histidine-tag in the protein sequence. In addition, Anti-His₆ preferentially recognizes the C-terminal His₆-epitope with high sensitivity. Anti-His₆ allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.

The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.

Preparation Note

Stabilizers: BSA
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

• ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml
• Immunoprecipitation: 0.5 to 5 μg/ml
• Western blot: 0.2 to 0.5 μg/ml

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317,H412
• pcodes: P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash