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SAB4200476

Sigma-Aldrich

Anti-ALIX antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-AIP1, Anti-ALG-2 interacting protein 1, Anti-DRIP4, Anti-HP95, Anti-PDCD6IP, Anti-apoptosis-linked gene 2-interacting protein X, Anti-dopamine receptor interacting protein 4, Anti-programmed cell death 6 interacting protein
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

species reactivity

mouse, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of human Jurkat cells.
western blot: 2.5-5 μg/mL using whole extracts of mouse A20 cells.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PDCD6IP(10015)
mouse ... Pdcd6ip(18571)

Related Categories

General description

The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region.

Immunogen

peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in monkey, bovine and dog and differs by 2 amino acids in mouse and rat.

Application

Anti-ALIX antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X binds to endophilins, proteins that regulate membrane shape during endocytosis. Over-expression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.
PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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