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Key Documents

T5701

Sigma-Aldrich

Anti-TSG101 antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Tumor Suspectibility Gene 101

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 46 kDa

species reactivity

rat, mouse, human

concentration

~2 mg/mL

technique(s)

western blot: 0.5-1 μg/mL using cell lysates of rat fibroblast Rat-1 and mouse fibroblast NIH3T3 cells
western blot: 2-4 μg/mL using cell lysates of human A431 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TSG101(7251)
mouse ... Tsg101(22088)
rat ... Tsg101(292925)

General description

Tumor susceptibility 101 (TSG101) is encoded by the gene mapped to human chromosome 11p15.1–15.2. The encoded protein is a member of catalytically inactive ubiquitin E2 variant (UEV) family. Tsg101 is characterized with an ubiquitin E2 variant (UEV) domain in its N-terminal end involved in HIV-1 budding and the vacuolar protein sorting (VPS) pathway.

Immunogen

syntheitic peptide corresponding to amino acids 254-272 located near the C-terminus of human TSG101, conjugated to KLH. This sequence is identical in rat and mouse TSG101.

Application

Anti-TSG101 antibody produced in rabbit has been used in western blot analysis.

Biochem/physiol Actions

Tumor susceptibility 101 (TSG101) is implicated in various cellular function such as, ubiquitination, transcriptional regulation, endosomal trafficking and cell proliferation. Mutation in the gene leads to various human cancers.Tsg101 plays a crucial role in cell growth, cell survival, and normal function of embryonic and adult tissues. TSG101 facilitates infectious human immunodeficiency virus (HIV-1) budding by linking the p6 late domain to vacuolar protein sorting machinery. Tsg101 is a vital constituent of the endosomal sorting complex required for transport (ESCRT) machinery, that helps in synthesis of multi-vesicular bodies, mainly the formation and scission of the intraluminal vesicles, and is thus, crucial for the degradation and recycling of receptors localized on plasma membrane. Tsg101 expression is controlled by an intrinsic autoregulatory mechanism and by ubiquitination catalyzed by various ubiquitin ligases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

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Owen Pornillos et al.
The EMBO journal, 21(10), 2397-2406 (2002-05-15)
Human Tsg101 plays key roles in HIV budding and in cellular vacuolar protein sorting (VPS). In performing these functions, Tsg101 binds both ubiquitin (Ub) and the PTAP tetrapeptide 'late domain' motif located within the viral Gag protein. These interactions are
Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum
Tang YT, et al.
International Journal of Molecular Medicine, 40(3), 834-844 (2017)
Kay-Uwe Wagner et al.
Molecular and cellular biology, 23(1), 150-162 (2002-12-17)
Tumor susceptibility gene 101 (Tsg101) was identified in a random mutagenesis screen for potential tumor suppressors in NIH 3T3 cells. Altered transcripts of this gene have been detected in sporadic breast cancers and many other human malignancies. However, the involvement
Cancer-testis antigens MAGEA proteins are incorporated into extracellular vesicles released by cells
Kuldkepp A, et al.
Oncotarget, 10(38), 3694-3694 (2019)
James V McCann et al.
Scientific reports, 9(1), 16743-16743 (2019-11-16)
Extracellular vesicles (EVs) play important roles in tumor progression by altering immune surveillance, promoting vascular dysfunction, and priming distant sites for organotropic metastases. The miRNA expression patterns in circulating EVs are important diagnostic tools in cancer. However, multiple cell types

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