SAB4200699
Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal
clone TH-2, purified from hybridoma cell culture
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All Photos(1)
Synonym(s):
TH, TYH
UNSPSC Code:
12352203
NACRES:
NA.44
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TH-2, monoclonal
form
buffered aqueous solution
species reactivity
rabbit, human, sheep, guinea pig, rat, bovine, mouse
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 0.25-0.5 μg/mL using whole extracts of rat PC-12 cells.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TH(7054)
General description
Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TH-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat Tyrosine Hydroxylase. Tyrosine Hydroxylase (TH) also known as tyrosine 3- monooxygenase, is encoded by the gene mapped to human chromosome 11p15.5. It is a tetrameric enzyme composed of four subunits. Four TH Isoforms were identified in humans.
Immunogen
purified rat Tyrosine Hydroxylase
Application
Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal has been used in various immunochemical techniques including immunoblotting, immunohistochemistry, immunofluorescence and immunoprecipitation.
Biochem/physiol Actions
Tyrosine Hydroxylase (TH) catalyzes the hydroxylation of L-tyrosine to L-3,4-dihydroxyphenylalanine (L-dopa) in brain and adrenal medulla which is the initial and rate limiting step of the neurotransmitters and hormones catecholamines (dopamine, norepinephrine and epinephrine) biosynthesis. Therefore, TH plays a key role in the physiology of adrenergic neurons. Tyrosine Hydroxylase (TH) deficiency is associated with the development of wide variety of phenotypic spectrum such as several types of dystonia, early-onset of Parkinson′s, cerebral palsy or spastic paraplegia and both primary and secondary deficiencies of cerebrospinal fluid (CSF) neurotransmitter metabolites.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Tyrosine hydroxylase deficiency
GeneReviews(?) (2017)
Species differences in the expression of multiple tyrosine hydroxylase protein isoforms
Haycock J W
Journal of Neurochemistry, 81, 947-953 (2002)
Tyrosine Hydroxylase and Regulation of Dopamine Synthesis
Daubner SC, et al.
Archives of Biochemistry and Biophysics, 508, 1-1 (2011)
Chunhui Yang et al.
Aging, 12(16), 16062-16071 (2020-08-25)
Electroconvulsive therapy (ECT) has known beneficial effects on the core motor symptoms of Parkinson's disease (PD), likely through induction of dopamine release and sensitivity of dopamine receptors. Mesenchymal stem cells (MSCs) can salvage loss of dopamine in PD through their
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