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SAB4200705

Sigma-Aldrich

Anti-Neurofilament 200 (Phos and Non-Phos) antibody, Mouse monoclonal

clone N52, purified from hybridoma cell culture

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Synonym(s):
Anti-CMT2CC, Anti-NFH
NACRES:
NA.41

antibody form

purified from hybridoma cell culture

Quality Level

antibody product type

primary antibodies

clone

N52, monoclonal

mol wt

~200 kDa

species reactivity

mouse, pig, feline, rat, monkey, bovine, human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2.5-5 μg/mL using human neuroblastoma SH-SY5Y cell line fresh lysate
immunofluorescence: suitable
immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Cerebellum sections

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
N0142SAB4200747N5389
clone

N52, monoclonal

clone

N52, monoclonal

clone

NE14, monoclonal

clone

NE14, monoclonal

mol wt

~200 kDa

mol wt

-

mol wt

~200 kDa

mol wt

antigen apparent mol wt 200 kDa

species reactivity

mouse, pig, feline, rat, monkey, bovine, human

species reactivity

wide range

species reactivity

feline, rat, guinea pig, pig, human, mouse, bovine, chicken

species reactivity

pig, mouse, chicken, bovine, guinea pig, rabbit, rat, human

concentration

~1.0 mg/mL

concentration

-

concentration

~1.0 mg/mL

concentration

-

technique(s)

immunoblotting: 2.5-5 μg/mL using human neuroblastoma SH-SY5Y cell line fresh lysate, immunofluorescence: suitable, immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Cerebellum sections

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): 1:400 using rat cerebellum, microarray: suitable, western blot: 1:1,000 using rat brain extract

technique(s)

immunoblotting: 1.25-2.5 μg/mL using rat brain S1 fraction, immunohistochemistry: 5-10 μg/mL using enzyme treated formalin-fixed, paraffin-embedded rat brain or mouse brain sections

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, immunohistochemistry (frozen sections): suitable, microarray: suitable, western blot: suitable

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General description

Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the N52 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilament 200 also known as Neurofilament heavy polypeptide (H-subunit) and is 200 kDa.
Neurofilament 200 is encoded by the gene located at human chromosome 22q12.2.

Specificity

Monoclonal Anti-Neurofilament 200 (Phos. and NonPhos.) also known as Neurofilament-H or Heavy subunit, specifically recognizes an epitope on the C-terminal tail domain of Neurofilament 200, which is present on both the phosphorylated and nonphosphorylated forms of this glycoprotein. The antibody shows reactivity with neurofilaments in the central and peripheral nervous systems from human , pig, mouse, rat, monkey, feline and bovine origin.

Application

Anti-Neurofilament 200 (Phos and Non-Phos) antibody has been used in
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Mutation in the NEFH gene leads to the development of autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc).
Neurofilament 200 has an important function in axonal transport, axonal plasticity and maintaining the structure of neuronal cytoskeleton. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Alterations of a 200 kDa neurofilament in the rat hippocampus after forebrain ischemia
Kaku Y, et al.
Journal of Cerebral Blood Flow and Metabolism, 13(3), 402-408 (1993)
Natalia Dubrowinskaja et al.
Cancer medicine, 3(2), 300-309 (2014-01-28)
Neurofilament Heavy polypeptid (NEFH) belongs to the group of type IV intermediate filament proteins. DNA methylation of the NEFH promoter and loss of expression have previously been shown to activate the AKT/β-catenin pathway in tumor cells. When identifying hypermethylation of
Dissociation of axonal neurofilament content from its transport rate
Yuan A, et al.
Testing, 10(7), e0133848-e0133848 (2015)
Kenji Kashiwagi et al.
Investigative ophthalmology & visual science, 44(1), 154-159 (2002-12-31)
To investigate the phosphorylation of the heavy neurofilament subunit (NF-H), which could be deeply involved in axonal transport of retinal ganglion cells (RGCs), in an experimental glaucoma model of chronic elevation of intraocular pressure (IOP) in monkeys. One eye in
Zhou Fang et al.
Frontiers in cellular neuroscience, 15, 816781-816781 (2022-01-11)
Collagen VI (COL6) in the microenvironment was recently identified as an extracellular signal that bears the function of promoting orderly axon bundle formation. However, the large molecular weight of COL6 (≈2,000 kDa) limits its production and clinical application. It remains

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