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SAB4200775

Sigma-Aldrich

Anti-Glycoprotein (MDR) antibody, Mouse monoclonal

clone F4, purified from hybridoma cell culture

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Synonym(s):
ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

F4, monoclonal

form

buffered aqueous solution

species reactivity

hamster, human

concentration

~1 mg/mL

technique(s)

flow cytometry: suitable
immunoblotting: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABCB1(5243)

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This Item
P7965M2444MAB4334
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

F4, monoclonal

clone

F4, monoclonal

clone

MDC1-50, monoclonal

clone

UIC2, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

species reactivity

hamster, human

species reactivity

hamster, human

species reactivity

monkey, human

species reactivity

primate, human

UniProt accession no.

P08183

UniProt accession no.

P08183

UniProt accession no.

Q14676

UniProt accession no.

P08183

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General description

Anti-P-Glycoprotein (MDR) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma F4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Multidrug resistance protein 1 (MDR1) is a member of the adenosine triphosphate (ATP)-binding cassette (ABC) transporters superfamily. It is ubiquitously expressed in kidneys, intestines, placenta, liver, adrenal glands blood-brain barrier (BBB), placenta and testis.

Specificity

Anti-P-Glycoprotein (MDR) antibody, Mouse monoclonal recognizes an epitope located at the N-terminal region of P-glycoprotein (Pgp), at the third extracellular loop of the molecule, also known as ABCB1 protein. The antibody specifically detects MDR1 P-glycoprotein, but does not recognize MDR3. Anti-P-Glycoprotein (MDR) cross reacts with MDR1 P-glycoprotein from human and hamster origin.

Immunogen

a mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Application

Anti-Glycoprotein (MDR) antibody, mouse monoclonal may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunohistochemistry
  • cellular enzyme-linked immunosorbent assay (ELISA)
  • flow cytometry (FACS)
  • cell surface radioimmunoassay (RIA)

Biochem/physiol Actions

Multidrug resistance protein 1 (MDR1) functions as an adenosine triphosphate (ATP)-dependent efflux pump. It is also involved in extruding certain xenobiotics and protect cells from toxicants. MDR1 gene is overexpressed in different cancer types including leukemias, lymphomas, myelomas, breast cancer, ovarian cancer, gastrointestinal stromal tumor (GIST), non-small cell lung cancer (NSCLC), fallopian tube, colon, renal, and thyroid cancers. Overexpression of MDR1 gene plays an important role in prompting multidrug resistance in cancer chemotherapy causing high resistance of cancerous cells to a wide variety of substrate anticancer drugs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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MDR1 genotype-related pharmacokinetics and pharmacodynamics
Sakaeda T, et al.
Biological & Pharmaceutical Bulletin, 25(11), 1391-1400 (2002)
Multidrug resistance in breast cancer: a meta-analysis of MDR1/gp170 expression and its possible functional significance
Trock B J, et al.
Journal of the National Cancer Institute, 89(13), 917-931 (1997)
Xing-Duo Dong et al.
Frontiers in pharmacology, 13, 843829-843829 (2022-03-15)
The KRAS-G12C inhibitor ARS-1620, is a novel specific covalent inhibitor of KRAS-G12C, possessing a strong targeting inhibitory effect on KRAS-G12C mutant tumors. Overexpression of ATP-binding cassette super-family B member 1 (ABCB1/P-gp) is one of the pivotal factors contributing to multidrug
ATP hydrolysis-dependent multidrug efflux transporter: MDR1/P-glycoprotein.
Kimura Y, et al.
Current Drug Metabolism, 5(1), 1-1 (2004)
Overexpression of ABCB1 Transporter Confers Resistance to mTOR Inhibitor WYE-354 in Cancer Cells
Wang J, et al.
International Journal of Molecular Sciences, 21(4), 1387-1387 (2020)

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