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MAB4334

Sigma-Aldrich

Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2

clone UIC2, Chemicon®, from mouse

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Synonym(s):
P-glycoprotein, CD243, p170, Pgp
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

UIC2, monoclonal

species reactivity

primate, human

should not react with

rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... ABCB1(5243)

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This Item
MAB4334ZMAB4162MAB4120
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

culture supernatant

clone

UIC2, monoclonal

clone

UIC2, monoclonal

clone

MM4.17, monoclonal

clone

JSB-1, monoclonal

species reactivity

primate, human

species reactivity

primate, human

species reactivity

human

species reactivity

human, hamster

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

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General description

UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE′s UIC2 Shift assay - see product ECM905).

Specificity

P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)

Immunogen

Epitope: conformational extracellular epitope
Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.

Application

Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2 detects level of MDR1 & has been published & validated for use in FC, IF, IP, IH, IH(P).
Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems.

Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.

Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.

Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.

IHC on frozen and paraffin sections: 1 - 10 μg/mL.

Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Toxicology & Drug Resistance

Physical form

Format: Purified
The immunoglobulin was purified by protein A Sepharose chromatography and is presented as a liquid in in 0.1M PBS, containing 0.1% sodium azide as a preservative.

Storage and Stability

Maintain at 2-8°C for up 12 months from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sarah M Valencia et al.
Journal of virology, 86(1), 2-10 (2011-10-28)
Epstein-Barr virus (EBV) uses different virus and cell proteins to enter its two major targets, B lymphocytes and epithelial cells. The routes that the virus takes into the two cell types are also different. To determine if these differences extend
Marzena Szwed et al.
Cellular oncology (Dordrecht), 37(6), 421-428 (2014-11-21)
Conjugation of anti-neoplastic agents with human proteins is a strategy to diminish the toxic side effects of anthracycline antibiotics. We have developed a novel doxorubicin-transferrin (DOX-TRF) conjugate aimed to direct anticancer drugs against therapeutic targets that display altered levels of
Valentina Fonsato et al.
Stem cells (Dayton, Ohio), 30(9), 1985-1998 (2012-06-28)
Microvesicles (MVs) play a pivotal role in cell-to-cell communication. Recent studies demonstrated that MVs may transfer genetic information between cells. Here, we show that MVs derived from human adult liver stem cells (HLSC) may reprogram in vitro HepG2 hepatoma and
Narasimha M Midde et al.
PloS one, 12(2), e0172628-e0172628 (2017-02-24)
Ethanol consumption is negatively associated with antiretroviral therapy (ART) adherence and general health in HIV positive individuals. Previously, we demonstrated ethanol-mediated alterations to metabolism of elvitegravir (EVG) in human liver microsomes. In the current study, we investigated ethanol influence on
Activity of the Bcl-2 family inhibitor ABT-263 in a panel of small cell lung cancer xenograft models.
Shoemaker, AR; Mitten, MJ; Adickes, J; Ackler, S; Refici, M; Ferguson, D; Oleksijew et al.
Clinical cancer research : an official journal of the American Association for Cancer Research null

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