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MAB4120

Sigma-Aldrich

Anti-MDR1 Antibody, clone JSB-1

culture supernatant, clone JSB-1, Chemicon®

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Synonym(s):
P-glycoprotein, CD243, p170, Pgp
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

JSB-1, monoclonal

species reactivity

human, hamster

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... ABCB1(5243)

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This Item
MAB4163M7317SAB4700911
antibody form

culture supernatant

antibody form

purified immunoglobulin

antibody form

tissue culture supernatant

antibody form

purified immunoglobulin

clone

JSB-1, monoclonal

clone

MC57, monoclonal

clone

P3II-26, monoclonal

clone

UIC2, monoclonal

species reactivity

human, hamster

species reactivity

human

species reactivity

human

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

immunocytochemistry: 1:20-1:50 using acetone-fixed cytospin preparations, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (not suitable for human tissues), immunohistochemistry (frozen sections): 1:20 using acetone-fixed sections, western blot: suitable

technique(s)

flow cytometry: 2 μg/mL

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Specificity

Reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170 kDa P-gp, member of the superfamily of transmembrane transporters. JSB-1 detects P-gp overexpression in human tumor cells of all different histogenetic derivations.

JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.

Immunogen

Epitope: Cytoplasmic

Application

Anti-MDR1 Antibody, clone JSB-1 detects level of MDR1 & has been published & validated for use in FC, IC, IH(P) & WB.
Flow Cytometry

Immunohistochemistry on frozen and paraffin embedded tissue

sections: 1:20.

Immunocytochemistry: acetone or air-dried preparations react well.

Western blot

Note: For cellular detection, permeabilization is required.

Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Toxicology & Drug Resistance

Target description

170 kDa

Physical form

UnPurified mouse culture supernatant containing 0.7% BSA and 0.1% sodium azide as a preservative.
Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
MDR cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Andrea Ries Thurman et al.
Journal of acquired immune deficiency syndromes (1999), 78(1), 82-92 (2018-02-10)
We describe and compare the local and systemic pharmacokinetics (PK) of tenofovir (TFV) and TFV-diphosphate (TFV-DP) in healthy premenopausal (PRE) and postmenopausal (POST) women using TFV 1% gel and correlate local PK with other mucosal end points. PRE (n =
Matthew W Wilson et al.
Investigative ophthalmology & visual science, 47(4), 1269-1273 (2006-03-28)
To compare the expression of multidrug-resistant proteins in retinoblastoma tumors among eyes treated with primary enucleation. A group of 18 patients with unilateral retinoblastoma with advanced intraocular disease was selected for the study. All patients had undergone primary enucleation. A
Multidrug Resistance Gene (MDR-1) and Risk of Brain Metastasis in Epithelial Ovarian, Fallopian Tube, and Peritoneal Cancer.
Matsuo K, Eno ML, Ahn EH, Shahzad MM, Im DD, Rosenshein NB, Sood AK
American Journal of Clinical Oncology null
Daria Brambilla et al.
International journal of cancer, 130(12), 2824-2834 (2011-07-23)
Overexpression of the mdr1 gene encoding P-glycoprotein (Pgp) exerts a major role in reducing the effectiveness of cytotoxic therapy in osteosarcoma. The interaction between actin and Pgp has been shown to be instrumental in the establishment of multidrug resistance (MDR)
T Yanagisawa et al.
British journal of cancer, 80(8), 1190-1196 (1999-06-22)
Clinical studies have suggested that both MDR1 and MRP may play a significant role in the chemosensitivity and outcome of neuroblastoma. To clarify the nature of multidrug resistance (MDR) in this tumour a series of six neuroblastoma cell lines have

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