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SAB4200796

Sigma-Aldrich

Anti- Bovine IgG-Peroxidase antibody, Mouse monoclonal

clone BG-18, purified from hybridoma cell culture

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Synonym(s):
Bovine immunoglobulin G

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

BG-18, monoclonal

form

lyophilized powder

species reactivity

bovine

concentration

~2 mg/mL

technique(s)

direct ELISA: 1:20,000-1:40,000 using 1 μg/ml bovine IgG for coating

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
SAB4200768SAB4200755SAB4200748
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

BG-18, monoclonal

clone

8c/6-39, monoclonal

clone

GE-1, monoclonal

clone

MB-11, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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General description

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the BG-18 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the purified bovine IgG.
Immunoglobulin G (IgG) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.
The bovine immunoglobulin system closely resembles that of other mammalian species with respect to the physiochemical properties and nomenclature. The main bovine IgG class antibodies are IgG1 and IgG2, which have antigenic differences in the Fc region of the heavy chains.4 

Specificity

Monoclonal Anti-Bovine IgG specifically recognizes an epitope on the heavy chain of both bovine IgG1 and IgG2. The antibody shows no cross-reactivity with bovine IgM or IgG from the following species: human, goat, sheep, rabbit, pig, dog, chicken, cat, guinea pig or horse.

Immunogen

Purified bovine IgG

Application

The antibody is recommended to use in various immunological techniques, including ELISA1, Immunohistochemistry2 and Immunobloting3

Biochem/physiol Actions

Different immunoglobulin classes and subclasses (isotypes) perform distinctive effector functions. Therefore, the ability to characterize antibody isotype is fundamental to the analysis of the immune response. The diversity of immunoglobulin isotypes associated with the immunity pattern serves as an indicator indistinguishing the phase of infection with various agents.

Physical form

Supplied as a lyophilized powder.

Storage and Stability

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Gestur Vidarsson et al.
Frontiers in immunology, 5, 520-520 (2014-11-05)
Of the five immunoglobulin isotypes, immunoglobulin G (IgG) is most abundant in human serum. The four subclasses, IgG1, IgG2, IgG3, and IgG4, which are highly conserved, differ in their constant region, particularly in their hinges and upper CH2 domains. These

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