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SAB4200798

Sigma-Aldrich

Anti-GSDMD antibody, Mouse monoclonal

clone GSD-14, purified from hybridoma cell culture

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Synonym(s):
Anti-Gasdermin domain-containing protein 1, Anti-Gasdermin-D
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GSD-14, monoclonal

form

buffered aqueous solution

mol wt

~53 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using human Jurkat cell extract
immunofluorescence: 5-10 μg/mL using human A431 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GSDMD(79792)

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This Item
G7422WH0079792M1SAB4200473
antibody form

purified from hybridoma cell culture

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

clone

GSD-14, monoclonal

clone

polyclonal

clone

3F12-1B2, monoclonal

clone

4A7, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

~53 kDa

mol wt

antigen ~55 kDa

mol wt

-

mol wt

antigen ~40 kDa

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, mouse

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General description

GSDMD (gasdermin D) also known as DFNA5L or Gasdermin domain-containing 1 (GSDMDC1), belongs to the gasdermin protein family of epithelial proliferation regulators. GSDMD is expressed in the upper gastrointestinal epithelium differentiating cells and has been suggested to act as a tumor suppressor since it is suppressed in high percentage of esophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gramnegative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1β processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). It was also reported that Caspase-4/11 specifically cleaves GSDMD after Asp275, leading to gasdermin-N domain that bears intrinsic pyroptosisinducing activity. 4 Furthermore, GSDMD-mediated pyroptosis plays an important role in mature IL-1β release without affecting its maturation.

Monoclonal Anti-GSDMD recognizes human GSDMD.
Monoclonal Anti-GSDMD (mouse IgG1 isotype) is derived from the GSD-14hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with synthetic peptide corresponding to the internal region of human GSDMD, conjugated to keyhole limpet hemocyanine (KLH). Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1). It belongs to the gasdermin protein family of epithelial proliferation regulators and is expressed in the upper gastrointestinal epithelium differentiating cells.

Immunogen

Synthetic peptide of human GSDMD, conjugated to KLH

Application

The antibody is recommended to use in various immunological techniques, including Immunoblot (∼53 kDa) and Immunofluorescence.

Biochem/physiol Actions

Gasdermin D (GSDMD) is known to act as a tumor suppressor since it is suppressed in a high percentage of oesophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gram-negative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1 processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). Furthermore, GSDMD-mediated pyroptosis plays an important role in mature interleukin-1 (IL-1) release without affecting its maturation.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Inflammatory caspase-related pyroptosis: mechanism, regulation and therapeutic potential for inflammatory bowel disease
Kayagaki N, et al.
Gastroenterology, 526(7575), 666-666 (2015)
Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling
Kayagaki N, et al.
Nature, 526(7575), 666-666 (2015)
Wan-ting He et al.
Cell research, 25(12), 1285-1298 (2015-11-28)
Inflammasome is an intracellular signaling complex of the innate immune system. Activation of inflammasomes promotes the secretion of interleukin 1β (IL-1β) and IL-18 and triggers pyroptosis. Caspase-1 and -11 (or -4/5 in human) in the canonical and non-canonical inflammasome pathways
Members of a novel gene family, Gsdm, are expressed exclusively in the epithelium of the skin and gastrointestinal tract in a highly tissue-specific manner
Tamura M, et al.
Genomics, 89(5), 618-629 (2007)
Caina Ma et al.
Virologica Sinica, 37(3), 445-454 (2022-05-06)
African swine fever (ASF) is a highly pathogenic swine infectious disease that affects domestic pigs and wild boar, which is caused by the African swine fever virus (ASFV). ASF has caused huge economic losses to the pig industry and seriously

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