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SAB4600371

Sigma-Aldrich

Monoclonal Anti-6X His IgG, CF680 antibody produced in mouse

~2 mg/mL, clone 33D10.D2.G8, affinity isolated antibody, buffered aqueous solution

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NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

CF 680 conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

33D10.D2.G8, monoclonal

form

buffered aqueous solution

concentration

~2 mg/mL

technique(s)

indirect immunofluorescence: 1-10 μg/mL
western blot: suitable

isotype

IgG1κ

fluorescence

λex 681 nm; λem 698 nm

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3 and IgG4 with different heavy chains, named γ1,γ2, γ3 and γ4, respectively. IgG is produced and released from CD5+B cells, the predominant lymphocytes in the neonatal cell repertoire. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes infants.
This affinity purified antibody is directed against the 6X HIS epitope and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the 6X HIS epitope tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. This product is prepared by labeling high quality monoclonal mouse anti-6X His IgG(H+L) with our near-IR fluorescent dye CF680.CF680 is one of our three outstanding near-IR CF dyes developed by Biotium. Near-IR CF dyes are superior to other similar near-IR dyes, such as Alexa Fluor dyes, IRDyes and the DyLight near-IR dyes. Near-IR CF dyes have combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging, near-IR Western blotting and flow cytometry.

Specificity

Binds 6X-His epitope tag

Immunogen

proteins contatining the epitope tag sequence

Application

Monoclonal Anti-6X His IgG, CF 680 antibody produced in mouse has been used in western blot.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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