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SAE0097

Sigma-Aldrich

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) from Acidaminococcus fermentans

recombinant, expressed in E. coli, aqueous solution

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Synonym(s):
D2HGDH, HGDH, L-2-hydroxyglutarate dehydrogenase
Enzyme Commission number:
1.1. 99.2

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

aqueous solution

specific activity

≥1000 units/mg protein

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

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This Item
G6751L2011K1502
specific activity

≥1000 units/mg protein

specific activity

100-300 units/mg protein

specific activity

≥250 units/mg protein (biuret)

specific activity

0.1-1.0 units/mg protein (Lowry)

form

aqueous solution

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

buffered aqueous glycerol solution

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

UniProt accession no.

D2RJU7

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

General description

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) is a member of the D-2-hydroxyacid NAD+ dependent dehydrogenase family of proteins. D2HGDH catalyzes the conversion of α-ketoglutarate (α--KG) to D-2-hydroxyglutarate (D2HG), coupled to the oxidation of NADH to NAD+ .

The crystal structure of D2HGDH from Acidaminococcus fermentans has been reported. D2HGDH from Acidaminococcus fermentans has been used in several enzymatic assays, such as:
  • A continuous spectrophotometric assay to measure the activity of aminotransferases, based on the transamination of a keto compound and L-glutamate, which yields a corresponding amino compound and 2-oxoglutarate.
  • Determination of D2HG levels in biological fluids such as serum, urine, cell culture supernatants, and cell or tissue lysates.
  • A coupled assay system to measure branched-chain amino acid aminotransferase activity.

Unit Definition

One unit of enzyme oxidizes 1 μmole of NADH to NAD+ coupled to the reduction of α-ketoglutarate to (D)-2-hydroxyglutarate per minute at 37°C at pH 8.0.

Preparation Note

This recombinant D2HGDH product is supplied as an aqueous solution in 20 mM Trizma® buffer, pH 7.5, with 150 mM NaCl, and 10% glycerol.

Legal Information

T3P is a registered trademark of Archimica GmbH

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

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1 of 5

Berta M Martins et al.
The FEBS journal, 272(1), 269-281 (2005-01-07)
NAD(+)-dependent (R)-2-hydroxyglutarate dehydrogenase (HGDH) catalyses the reduction of 2-oxoglutarate to (R)-2-hydroxyglutarate and belongs to the d-2-hydroxyacid NAD(+)-dependent dehydrogenase (d-2-hydroxyacid dehydrogenase) protein family. Its crystal structure was determined by phase combination to 1.98 A resolution. Structure-function relationships obtained by the comparison
Xuejing Yu et al.
Analytical biochemistry, 431(2), 127-131 (2012-09-25)
A continuous general spectrophotometric assay for measuring the activity of aminotransferases has been developed. It is based on the transamination of a keto compound (amino acceptor) and l-glutamate (amino donor), yielding the corresponding amino compound and 2-oxoglutarate. The rate of
Jörg Balss et al.
Acta neuropathologica, 124(6), 883-891 (2012-11-03)
Levels of (D)-2-hydroxyglutarate [D2HG, (R)-2-hydroxyglutarate] are increased in some metabolic diseases and in neoplasms with mutations in the isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) genes. Determination of D2HG is of relevance to diagnosis and monitoring of disease.
Xuejing Yu et al.
The FEBS journal, 281(1), 391-400 (2013-11-12)
Branched-chain amino acid aminotransferase (BCAT) plays a key role in the biosynthesis of hydrophobic amino acids (such as leucine, isoleucine and valine), and its substrate spectrum has not been fully explored or exploited owing to the inescapable restrictions of previous

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