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SAE0107

Sigma-Aldrich

3-Methyladenine Ready made solution

≥98% (HPLC), 7.5mg/ml (50mM) in MES buffer, Autophagy inhibitor

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Synonym(s):
3-MA, 6-Amino-3-methylpurine, autophagy inhibitor

Assay

≥98% (HPLC)

form

liquid

concentration

7.5 mg/mL

shipped in

ambient

storage temp.

−20°C

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This Item
SML2959M5353SML3001
form

liquid

form

liquid

form

liquid

form

liquid

concentration

7.5 mg/mL

concentration

-

concentration

10 mg/mL in DMSO

concentration

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

3-Methyladenine (3-MA)  is a methylated derivative of adenine that has been used as an In vivo marker for the methylation of DNA. 3-Methyladenine and 7-methylguanine are the two main N-methylpurines formed in DNA upon exposure to methylating agents. 3-MA is removed from damaged DNA via the DNA base excision repair pathway, which begins with the enzyme N-methylpurine DNA glycosylase. The dependence of 3-MA repair on nucleotide excision repair in mammalian cell lines has been studied.3-MA have been widely used as autophagy inhibitor based on its inhibitory effect on class I and class III PI3K activity, which is known to be essential for induction of autophagy. 3-MA blocks class I PI3K persistently, whereas its suppressive effect on class III PI3K is transient, understanding the dual role of 3-MA in autophagy thus suggests that caution should be exercised in the application of 3-MA in autophagy study.
Furthermore, 3-MA is also capable of making the reduction of lymphocyte number caused by chronic stress rebound, implying that inhibition of autophagy can rescue chronic stress-induced immunosuppression.
Autophagy has been reported to play an important role in the 3, 4-Methylenedioxymethamphetamine MDMA-induced neurotoxicity in the mixed neuronal-glial primary culture and it was found that 3-MA ameliorate MDMA-induced autophagy, SERT loss, HPA axis dysfunction, and depressive behaviors.

Application

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5mM.

Preparation Note

3-Methyladenine ready made solution is provided at 50mM (7.5mg/mL) concentration in MES buffer.
It is recommended to store 3-methyladenine readymade solution at -20C.
In case percipitates are formed upon thaw, use sonication and heat to get the product back into solution.
It is recommended to avoid freeze thaw cycles.  
When stored properly 3-Methyladenine readymade solution should be stable for 2 years.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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