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SAE0203

Sigma-Aldrich

Anti-V5 Magnetic Beads

Magnetic agarose, Suspension

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Synonym(s):
Anti-V5 Tag antibody produced in mouse, V5 Antibody, V5 Tag Antibody, V5 epitope Tag

conjugate

magnetic beads

Quality Level

form

suspension

shipped in

ambient

storage temp.

2-8°C

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This Item
SAE0197V2260V8012
Anti-V5 Magnetic Beads Magnetic agarose, Suspension

SAE0203

Anti-V5 Magnetic Beads

Anti-HA Magnetic Beads Magnetic agarose, suspension

SAE0197

Anti-HA Magnetic Beads

Anti-V5 antibody, Mouse monoclonal clone V5-10, purified from hybridoma cell culture

V8012

Anti-V5 antibody, Mouse monoclonal

shipped in

ambient

shipped in

wet ice

shipped in

-

shipped in

dry ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

form

suspension

form

suspension

form

lyophilized powder

form

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

General description

The Anti-V5 Magnetic Beads are prepared using an affinity-purified anti-V5 antibody produced in mouse conjugated to cyanogen bromide-activated magnetic beads at a protein-to-bead ratio of approximately 2 mg/mL. The Anti-V5 antibody specifically recognizes an epitope located on V5-tagged fusion proteins. It offers specific reactivity with both N-terminal and C-terminal V5-tagged fusion proteins and is suitable for immunoprecipitation or immunoaffinity purification. These magnetic beads can be used for purifying expressed V5-tagged fusion proteins from bacterial lysates, mammalian lysates, or transfected cells and to study protein topology, characterize, and identify new proteins, protein complexes, and associated proteins.

Application

Anti-V5 magnetic beads have been used for the purification of V5-tagged proteins using the process of immunoprecipitation.

Features and Benefits

  • Beads are prepared with an affinity-purified anti-V5 antibody.
  • Monoclonal Anti-V5 antibody is produced by fusing mouse myeloma cells and splenocytes from a BALB/c mouse.
  • Protein-to-bead ratio is approximately 2 mg/mL.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Mitochondrial amidoxime-reducing component 1 p.Ala165Thr increases protein degradation mediated by the proteasome
Tanmoy Dutta et al.
Liver International : Official Journal of the International Association For the Study of the Liver (2024)

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