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V8012

Sigma-Aldrich

Anti-V5 antibody, Mouse monoclonal

clone V5-10, purified from hybridoma cell culture

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Synonym(s):
V5 Tag Monoclonal Antibody, V5 antibody
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

V5-10, monoclonal

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL
western blot: 0.5-1 μg/mL

shipped in

dry ice

storage temp.

−20°C

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V8012

Anti-V5 antibody, Mouse monoclonal

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

agarose conjugate

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

V5-10, monoclonal

clone

V5-10, monoclonal

clone

V9, monoclonal

clone

V5-10, monoclonal

technique(s)

immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL

technique(s)

western blot: 1:4,000-1:8,000

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 25-50 μg/mL using human tissue, microarray: suitable, western blot: 0.2-0.5 μg/mL using human fibroblasts HS-68

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

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General description

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide conjugated to KLH.
The mouse monoclonal Anti-V5 antibody reacts specifically with the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.
The mouse mooclonal Anti-V5 antibody reacts specifically with the V5-taged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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