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V2260

Sigma-Aldrich

Monoclonal Anti-V5-Peroxidase antibody produced in mouse

clone V5-10, purified immunoglobulin, lyophilized powder

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Synonym(s):
Monoclonal Anti-V5
MDL number:
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V5-10, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:4,000-1:8,000

storage temp.

2-8°C

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This Item
V8012A7058A2074
vibrant-m

V8012

Anti-V5 antibody, Mouse monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

technique(s)

western blot: 1:4,000-1:8,000

technique(s)

immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue, western blot (chemiluminescent): 1:160,000

form

lyophilized powder

form

-

form

lyophilized powder

form

lyophilized powder

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General description

The V5 tag is a 14-amino acid long peptide sequence (GKPIPNPLLGLDST) that is derived from an epitope on the P and V proteins of simian virus 5 (SV5), which belongs to the paramyxovirus family. Monoclonal Anti-V5 Peroxidase conjugate reacts with V5-tagged recombinant fusion proteins expressed in transfected mmmalian cells or produced by in vitro translation.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Suitable for immunoblotting.

Physical form

Lyophilized from a 0.01 M phosphate buffered saline solution, pH 7.4, containing 1% BSA and 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Reconstitute with 0.5 mL of distilled water.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Panayotis C Theodoropoulos et al.
Nature chemical biology, 12(4), 218-225 (2016-02-02)
A hallmark of targeted cancer therapies is selective toxicity among cancer cell lines. We evaluated results from a viability screen of over 200,000 small molecules to identify two chemical series, oxalamides and benzothiazoles, that were selectively toxic at low nanomolar
Steven F Baker et al.
Cell reports, 24(10), 2581-2588 (2018-09-06)
Adaptation of viruses to their hosts can result in specialization and a restricted host range. Species-specific polymorphisms in the influenza virus polymerase restrict its host range during transmission from birds to mammals. ANP32A was recently identified as a cellular co-factor
Mauricio P Contreras et al.
Science advances, 9(18), eadg3861-eadg3861 (2023-05-03)
Parasites counteract host immunity by suppressing helper nucleotide binding and leucine-rich repeat (NLR) proteins that function as central nodes in immune receptor networks. Understanding the mechanisms of immunosuppression can lead to strategies for bioengineering disease resistance. Here, we show that
Vy Tran et al.
Nature microbiology, 5(12), 1490-1503 (2020-08-26)
Cells infected by influenza virus mount a large-scale antiviral response and most cells ultimately initiate cell-death pathways in an attempt to suppress viral replication. We performed a CRISPR-Cas9-knockout selection designed to identify host factors required for replication after viral entry.
Adriana López-Barradas et al.
American journal of physiology. Cell physiology, 311(5), C720-C734 (2016-11-03)
SMCTs move several important fuel molecules that are involved in lipid, carbohydrate, and amino acid metabolism, but their regulation has been poorly studied. Insulin controls the translocation of several solutes that are involved in energetic cellular metabolism, including glucose. We

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