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SCJ4600020

Sigma-Aldrich

CF 555 hydrazide

suitable for fluorescence

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NACRES:
NA.41

form

solid

Quality Level

mol wt

~3000 g/mol

ε (extinction coefficient)

150000 at 555 nm

absorbance ratio

A280/555 nm 0.08

fluorescence

λex 488 or 532 nm (Flow cytometry laser line)
λex 488 or 532 nm (Microscopy laser line)
λex 555 nm; λem 565 nm

suitability

suitable for fluorescence

storage temp.

−20°C

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CF™ 555 hydrazide suitable for fluorescence

SCJ4600020

CF 555 hydrazide

Sigma-Aldrich

SCJ4600019

CF 555 amine

Sigma-Aldrich

SCJ4600021

CF 555 maleimide

CF™ 647 hydrazide suitable for fluorescence

SCJ4600046

CF 647 hydrazide

fluorescence

λex 488 or 532 nm (Flow cytometry laser line), λex 555 nm; λem 565 nm, λex 488 or 532 nm (Microscopy laser line)

fluorescence

λex 488 or 532 nm (Flow cytometry laser line), λex 488 or 532 nm (Microscopy laser line), λex 555 nm; λem 565 nm

fluorescence

λex 488 or 532 nm (Flow cytometry laser line), λex 488 or 532 nm (Microscopy laser line), λex 555 nm; λem 565 nm

fluorescence

λex 633, 635 or 640 nm (Flow cytometry laser line), λex 633, 635 or 640 nm (Microscopy laser line), λex 650 nm; λem 665 nm

form

solid

form

solid

form

solid

form

solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

ε (extinction coefficient)

150000 at 555 nm

ε (extinction coefficient)

150000 at 555 nm

ε (extinction coefficient)

-

ε (extinction coefficient)

240000 at 650 nm

General description

CF 555 is a red fluorescent dye spectrally similar to Cy3 and Alexa Fluor 555. CF 555 also has comparable brightness and photostability as Cy 3 and Alexa Fluor 555.

CF dye hydrazides can be used as a fixable polar tracers for visualizing cell morphology,or for gap junction studies. The dyes can be introduced into cells by microinjection. For conjugation to aldehyde or ketone groups (such as carbohydrate molecules after peroxidation with periodate) we recommend using the more reactive CF aminooxy dye forms.

Features and Benefits

1. Has comparable brightness and photostability as Cy®3 and Alexa Fluor555 2. Highly water-soluble 3. pH-insensitive.

Preparation Note

Protect from light

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.
Cy is a registered trademark of Cytiva
Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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