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SHC016H

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer

Targets no known genes from any species

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Synonym(s):
MISSION®, MISSION® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
NACRES:
NA.51

Quality Level

product line

MISSION®

concentration

≥1x109 VP/ml (via p24 assay)

shipped in

dry ice

storage temp.

−70°C

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SHC016VSHC016SHC216V
product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Quality Level

200

Quality Level

100, 200

Quality Level

200

Quality Level

100, 200

General description

The MISSION pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
High titer virus is useful when performing in vivo research.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Najim Lahrouchi et al.
Nature communications, 10(1), 1180-1180 (2019-03-14)
A failure in optic fissure fusion during development can lead to blinding malformations of the eye. Here, we report a syndrome characterized by facial dysmorphism, colobomatous microphthalmia, ptosis and syndactyly with or without nephropathy, associated with homozygous frameshift mutations in

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