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SRE0026

Sigma-Aldrich

Phosphatase substrate

Suitable for manufacturing of diagnostic kits and reagents

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Synonym(s):
Phosphatase substrate, 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

≥97% (HPLC)

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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This Item
P5869S0942P5994
vibrant-m

SRE0026

Phosphatase substrate

vibrant-m

P5869

Phosphatase substrate

vibrant-m

S0942

Phosphatase substrate

vibrant-m

P5994

Phosphatase substrate

Quality Level

400

Quality Level

200

Quality Level

300

Quality Level

200

assay

≥97% (HPLC)

assay

-

assay

-

assay

-

form

powder

form

powder

form

tablet (round)

form

tablet

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

Application

Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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