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SRP0233

Sigma-Aldrich

PI3 kinase (p110a/p85a) Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

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Synonym(s):
PIK3CA, Phosphoinositide-3-kinase, catalytic, alpha, PtdIns-3-kinase p110
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

specific activity

≥13 pmol/min-μg

mol wt

129 kDa (p110α)
88 kDa (p85α)

packaging

pkg of 20 μg

concentration

>0.02 mg/mL

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PIK3CA(5290)

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SRP0249

PLK2 Active human

Gene Information

human ... PIK3CA(5290)

Gene Information

human ... PIK3CA(5290)

Gene Information

human ... PIK3CD(5293)

Gene Information

human ... PLK2(10769)

assay

≥80% (SDS-PAGE)

assay

≥60% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥60% (SDS-PAGE)

specific activity

≥13 pmol/min-μg

specific activity

≥1770 pmol/min-μg

specific activity

≥1.1 pmol/min-μg

specific activity

≥3.6 pmol/min-μg

NCBI accession no.

U79143 (p110alpha), XM_043865 (p85alpha)

NCBI accession no.

U79143 (p110alpha), XM_043865 (p85alpha)

NCBI accession no.

NM_005026 (p110delta), XM_043865 (p85alpha)

NCBI accession no.

NM_006622

UniProt accession no.

P42336

UniProt accession no.

P42336

UniProt accession no.

O00329

UniProt accession no.

Q9NYY3

General description

PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four subunits of the 110kDa catalytic subunit of PI3K protein, which contains a regulatory region of 85kDa. PI3K proteins form a family of lipid kinases. This gene is localized to human chromosome 3q.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four catalytic subunits of PI3K protein, which plays an essential role in extracellular growth signaling. It phosphorylates and activates protein kinase B (PKB) protein. This protein has a wide range of physiological functions, and plays central role in multiple types of cancers. PIK3CA gene is either mutated or amplified in brain, breast, stomach, liver, ovary and colon cancers. Mutation in this gene leads to enhanced PI3K activity, and it is a candidate transforming oncogene. It is over-expressed in invasive breast cancer and is a marker for poor prognosis. This gene is amplified in high-grade dysplastic lesions from bronchial biopsy specimens obtained from preinvasive squamous lung cancer. Frequency of mutations in PIK3CA gene is higher in tumors without lymph node metastasis, than those with in gastric cancer. Thus, it might be essential to evaluate the mutations in this gene to determine cetuximab treatment in gastric cancer patients.

Unit Definition

One unit of 110a/p85a kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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PIK3CA expression in invasive breast cancer: a biomarker of poor prognosis.
Aleskandarany MA, et al.
Breast Cancer Research and Treatment, 122(1), 45-53 (2010)
Progressive 3q amplification consistently targets SOX2 in preinvasive squamous lung cancer.
McCaughan F, et al.
American Journal of Respiratory and Critical Care Medicine, 182(1), 83-91 (2010)
Weipeng Lu et al.
Molecular medicine reports, 12(1), 1219-1224 (2015-03-31)
Cetuximab, an immunoglobulin G1 chimeric monoclonal antibody directed against the epidermal growth factor receptor, is currently considered to be the strategy with the most potential for the treatment of gastric cancer due to the low frequency of KRAS mutations in

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