SRP0234

PI3 kinase [p110a(E545K)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE)

• Synonym(s): PIK3CA, Phosphoinositide-3-kinase, catalytic, alpha, PtdIns-3-kinase p110
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• assay: ≥55% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥800 pmol/min-μg
• mol wt: 129 kDa; 88 kDa
• packaging: pkg of 20 μg
• concentration: 0.56 mg/mL
• NCBI accession no.: U79143 (p110alpha); XM_043865 (p85alpha)
• UniProt accession no.: P42336
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PIK3CA(5290)

Description

General description

Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1