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SRP0292

Sigma-Aldrich

Cathepsin S Active human

recombinant, expressed in FreeStyle 293-F cells, ≥90% (SDS-PAGE)

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Synonym(s):
MGC3886
NACRES:
NA.54

recombinant

expressed in FreeStyle 293-F cells

assay

≥90% (SDS-PAGE)

form

aqueous solution

specific activity

≥8100 pmol/min-μg

mol wt

37 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CTSS(1520)

General description

Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide(amino acids 1-16) + cathepsin S (amino acids 17-331), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.

Application

Active human cathepsin S is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin S has been used in a study to assess the pathogenesis of Alzheimer′s disease. Active human cathepsin S has also been used in a study to investigate the optimization of selectivity of Azepanone-based inhibitors.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 3 mM DTT, and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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