SRP0689

TAU-441, GSK3BETA-Phosphorylated

recombinant

• Synonym(s): Phosphorylated Tau Protein
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• recombinant: expressed in E. coli
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~64-68 kDa
• concentration: 0.2 μg/μL
• accession no.: P10636-8
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MAPT(4137)

Description

Biochem/physiol Actions

Recombinant human tag-free Tau-441 was co-expressed with GSK3beta in E. coli cells. The Tau-441 protein was phosphorylated by GSK3beta in vivo and in vitro prior to the final chromatography purification. Tau-441 or Tau-F is a member of the Tau family of proteins which function to stabilize the microtubules by binding to them. Tau proteins are subject to phosphorylation and this phenomenon regulates the association of the Tau protein with the microtubules ⁽¹⁾. Deposits of Alzheimer′s disease AD-associated proteins, such as hyperphosphorylated Tau, as well as other shared misfolded proteins, such as, beta-amyloid precursor protein (betaAPP), ubiquitin, and various chaperones and protein kinases are thought to play a pathologic role in the cognitive decline and muscular failure. Malfunctioning of Tau proteins is associated with microtubules disintegration and collapsing of the neuronal transport system ⁽²⁾.

Reconstitution

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable