SRP3285
GREMLIN-1 human
recombinant, expressed in CHO cells, ≥90% (SDS-PAGE), ≥90% (HPLC)
Synonym(s):
CKTSF1B1, DAND2, DRM
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
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biological source
human
recombinant
expressed in CHO cells
assay
≥90% (HPLC)
≥90% (SDS-PAGE)
form
lyophilized
potency
0.07-0.11 μg/mL
mol wt
13.7 kDa
packaging
pkg of 50 μg
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... GREM1(26585)
General description
Gremlin-1 (isoform-1) belongs to a group of diffusible proteins which bind to ligands of the transforming growth factor-β (TGF-β) family and regulate their activity by inhibiting their access to signaling receptors. The gene encoding this protein is localized on human chromosome 15q13.3. Recombinant Gremlin-1 is a 13.7kDa protein containing 160 amino acid residues.
Biochem/physiol Actions
The interplay between transforming growth factor-β (TGF-β) ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Gremlin is highly expressed in the small intestine, fetal brain, and colon and lower expression in brain, prostate, pancreas and skeletal muscle. Gremlin-1 regulates multiple functions in early development by specifically binding to and inhibiting the function of bone morphogenetic proteins -2,-4,-7 (BMP-2, -4, and -7). The protein has a role in limb and digit formation and in nephrogenesis. It also plays a role in carcinogenesis and kidney branching morphogenesis.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Common genetic variants at the CRAC1 (HMPS) locus on chromosome 15q13.3 influence colorectal cancer risk.
Jaeger E
Nature Genetics, 40(1), 26-28 (2008)
W Shi et al.
American journal of physiology. Lung cellular and molecular physiology, 280(5), L1030-L1039 (2001-04-06)
Bone morphogenetic protein-4 (BMP-4) is a key morphogen for embryonic lung development that is expressed at high levels in the peripheral epithelium, but the mechanisms that modulate BMP-4 function in early mouse lung branching morphogenesis are unclear. Here, we studied
Gremlin1 and chronic pancreatitis: a new clinical target and biomarker?
Brazil DP
Journal of Molecular Medicine, 93(10), 1057-1060 (2015)
Dong Qin et al.
Experimental and therapeutic medicine, 19(3), 2353-2359 (2020-02-28)
Proliferative vitreoretinopathy (PVR) is characterised by the contraction and growth of fibrotic membranes on the retina and within the vitreous body. Retinal pigment epithelial (RPE) cells, a major cellular component of the fibrotic membrane, is one of the cell types
Meta-analysis Reveals Genome-Wide Significance at 15q13 for Nonsyndromic Clefting of Both the Lip and the Palate, and Functional Analyses Implicate GREM1 As a Plausible Causative Gene.
Ludwig KU
PLoS Genetics, 12(3), e1005914-e1005914 (2016)
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