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SRP6054

Sigma-Aldrich

ProNGF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

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Synonym(s):
Beta-NGF, Beta-nerve growth factor
CAS Number:

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

~25 kDa

packaging

pkg of 10 μg

NCBI accession no.

shipped in

wet ice

Storage temp.

−20°C

Gene Information

human ... NGF(4803)

General description

The nerve growth factor (NGF) gene produces pro-nerve growth factor (proNGF), a soluble 246 amino acid pro-peptide. NGF belongs to the neurotrophin family. NGF gene is located on human chromosome 1p13.2.

Application

ProNGF human has been used as a standard in the de-glycosylation experiment for comparative studies by western blotting.

Biochem/physiol Actions

Pro-nerve growth factor (proNGF) participates in fetal neural development. It promotes neuronal survival and differentiation, or apoptosis, by interacting with particular nerve growth factor (NGF) receptors on neurons. Haploinsufficiency of the nerve growth factor β (NGFB) gene results in sensory neuropathy.
The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystine knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.

Physical form

Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.

Preparation Note

Centrifuge the vial prior to opening.

Reconstitution

Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.

Other Notes

Recombinant human ProNGF produced in E. coli is a non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met and having a molecular mass of 25 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Pro-His-Ser.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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