MilliporeSigma
All Photos(2)

Documents

T0678

Sigma-Aldrich

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse

clone WH-3, ascites fluid

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-TPH
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

WH-3, monoclonal

mol wt

antigen 55 kDa

contains

15 mM sodium azide

species reactivity

human, monkey, rabbit, rat

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using rabbit pineal gland extract

isotype

IgG3

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
T1299SAB2501377SAB4503029
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

WH-3, monoclonal

clone

TH-2, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, monkey, rabbit, rat

species reactivity

human, monkey, guinea pig, rat, sheep, bovine, rabbit

species reactivity

pig, canine, human, bovine

species reactivity

rat, mouse, human

biological source

mouse

biological source

mouse

biological source

goat

biological source

rabbit

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; L-tryptophan tetrahydropteridine:oxygen oxidoreductase; EC 1.14.16.4), the rate-limiting enzyme for the synthesis of serotonin, is a very specific enzyme found only inside serotoninergic neurons.
The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland.

Specificity

This antibody reacts specifically with tryptophan hydroxylase.

Immunogen

recombinant rabbit tryptophan hydroxylase.

Application

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunoblot
  • immunocytochemistry

Biochem/physiol Actions

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Brain tryptophan hydroxylase: purification of, production of antibodies to, and cellular and ultrastructural localization in serotonergic neurons of rat midbrain
Joh TH, et al.
Proceedings of the National Academy of Sciences of the USA, 72(9), 3575-3579 (1975)
Kadri Andre et al.
BMC psychiatry, 13, 118-118 (2013-04-20)
In major depression, one of the candidate genes possibly affecting the risk and severity of symptoms has been found to be tryptophan hydroxylase (TPH1). Variation in treatment response to antidepressive agents according to TPH1 genotype has also been found in
Honghai Zhang et al.
Epilepsia, 57(8), 1228-1235 (2016-06-16)
The DBA/1 mouse is a relevant animal model of sudden unexpected death in epilepsy (SUDEP), as it exhibits seizure-induced respiratory arrest (S-IRA) evoked by acoustic stimulation, followed by cardiac arrhythmia and death. Defects in serotonergic neurotransmission may contribute to S-IRA.
Anthony C Ruocco et al.
Neuropsychology, 30(1), 18-27 (2015-12-29)
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the formation of 5-hydroxytryptophan, a precursor to the neurotransmitter serotonin. Variations in the gene encoding this enzyme may underlie difficulties in impulse control; however, the proximate relationship between risk alleles for polymorphisms in
Neuronal loss in the rostral ventromedial medulla in a rat model of neuropathic pain
Leong ML, et al.
The Journal of Neuroscience, 31(47), 17028-17039 (2011)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service