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T2400

Sigma-Aldrich

Tropomyosin from porcine muscle

lyophilized powder (contains Tris buffer salts)

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CAS Number:
MDL number:
NACRES:
NA.77

biological source

Porcine muscle

Quality Level

assay

≥70% protein basis (biuret)

form

lyophilized powder (contains Tris buffer salts)

technique(s)

electrophoresis: suitable

storage temp.

−20°C

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This Item
A2522G1890G2500
Tropomyosin from porcine muscle lyophilized powder (contains Tris buffer salts)

T2400

Tropomyosin from porcine muscle

Sigma-Aldrich

A2522

Actin from rabbit muscle

Gelatin from porcine skin powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

G1890

Gelatin from porcine skin

Gelatin from porcine skin gel strength 300, Type A

G2500

Gelatin from porcine skin

technique(s)

electrophoresis: suitable

technique(s)

immunoblotting: suitable

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

biological source

Porcine muscle

biological source

rabbit

biological source

Porcine skin

biological source

Porcine skin

form

lyophilized powder (contains Tris buffer salts)

form

lyophilized powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Tropomyosin is a member of the actin filament binding protein family. It is a ubiquitous protein present in animals and fungi. Tropomyosin is made up of two α-helical chains arranged as a coiled-coil. It exists over twenty isoforms, in different eukaryotic cells like skeletal, cardiac, and smooth muscles, brain, platelets, fibroblasts, platelets, and other non-muscle cells.

Application

Tropomyosin from porcine muscle has been used:

  • as a positive control in actin- α-tropomyosin (TPM1) co-sedimentation analysis and assay
  • in analyzing the anti-adhesive properties of fish tropomyosins
  • in far Western blot analysis

Biochem/physiol Actions

Tropomyosin is associated with the actin cytoskeleton and plays a role in stabilizing and regulating the interactions of actin with other actin-binding proteins. It also plays a role in muscle contraction by regulating the interactions of actin with myosin.
Tropomyosin together with troponin, regulates the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help confer structure and function to actins in the cytoskeleton and striated muscle function. Dimeric protein that assumes an α-helical coiled-coil conformation. In the absence of Ca2+, it inhibits muscle contractility by blocking the myosin binding sites on actin.

Analysis Note

SDS electrophoresis shows two major bands.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Vertebrate tropomyosin: distribution, properties and function
Perry S V, et al.
Journal of Muscle Research and Cell Motility, 5?49-5?49 (2001)
A systematic nomenclature for mammalian tropomyosin isoforms
Michael A G, et al.
Journal of Muscle Research and Cell Motility, 147?153-147?153 (2015)
A novel alpha-tropomyosin mutation associates with dilated and non-compaction cardiomyopathy and diminishes actin binding
Judith B A, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1833(4), 833-839 (2013)
The molecular basis for tropomyosin isoform diversity
James P L M, et al.
Bioessays (1991)
M I Hamilton et al.
Clinical and experimental immunology, 99(3), 404-411 (1995-03-01)
Ulcerative colitis (UC) has a proposed autoimmune pathogenesis. A 40-kD antigen (P40) has been isolated from UC colon, bound to immunoglobulin. Tropomyosin has been reported as the target antigen of a MoAb (7E12H12) raised against P40. We set out to

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