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T5425

Sigma-Aldrich

Transforming Growth Factor-β3 human

TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture

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Synonym(s):
hTGF-β3, TGF-β3
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE)

form

powder

potency

0.01-0.09 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

predicted mol wt ~25 kDa

packaging

pkg of 2 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<0.01 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TGFB3(7043)

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H8541H8791T2815
Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

200

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in NSO cells

biological source

human

biological source

human

biological source

human

biological source

human

mol wt

predicted mol wt ~25 kDa

mol wt

dimer 25 kDa (non-glycosylated)

mol wt

dimer 25 kDa (non-glycosylated)

mol wt

25 kDa

General description

The TGFβ3 (transforming Growth Factor-β3) gene is mapped to human chromosome 14q24.3.

Application

Transforming Growth Factor-β3 human has been used to study the role of TGF-β3 in healing tendon-to-bone insertion and in chondrogenic differentiation.

Biochem/physiol Actions

TGF-β (transforming growth factor-β3) isoforms are significantly involved in inducing inflammatory responses. It is associated with vascular diseases, wound healing and ameliorating effect in venous leg ulcer. TGF-β3 has an antagonistic effect on TGF-β1 and reduces the degree of collagen type I deposition and fibroblast differentiation. As a result TGF-β3 might lead to ulcer chronicity due to hindered myofibroblasts formation. TGF-β is a key player in tumor formation and progression. Increased TGF-β3 expression is observed in cancer and adipose tissue fibrosis. TGF-β3 is crucial in tissue regeneration and scarless tissue repair.
TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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