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V4014

Sigma-Aldrich

Anti-V5-Cy3 antibody, Mouse monoclonal

purified immunoglobulin, ~1 mg/mL, clone V5-10

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Synonym(s):
Monoclonal Anti-V5
MDL number:
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V5-10, monoclonal

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 2.0-4.0 μg/mL

shipped in

wet ice

storage temp.

2-8°C

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This Item
V8012V2260C0992
Anti-V5 antibody, Mouse monoclonal clone V5-10, purified from hybridoma cell culture

V8012

Anti-V5 antibody, Mouse monoclonal

conjugate

CY3 conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

unconjugated

clone

V5-10, monoclonal

clone

V5-10, monoclonal

clone

V5-10, monoclonal

clone

CY-96, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

wet ice

shipped in

dry ice

shipped in

-

shipped in

dry ice

technique(s)

immunocytochemistry: 2.0-4.0 μg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL

technique(s)

western blot: 1:4,000-1:8,000

technique(s)

direct ELISA: suitable, dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable

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General description

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy3 is a trademark of Cytiva

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Dharmendra K Singh et al.
Molecular plant-microbe interactions : MPMI, 27(1), 7-17 (2013-09-21)
Protein kinase-driven phosphorylation constitutes the core of cellular signaling. Kinase components of signal transduction pathways are often targeted for inactivation by pathogens. The study of kinases and immune signal transduction in the model crop tomato (Solanum lycopersicum) would benefit from
J A Southern et al.
The Journal of general virology, 72 ( Pt 7), 1551-1557 (1991-07-01)
Two canine isolates of simian virus 5 (SV5), termed CPI+ and CPI-, were examined for their ability to react with a bank of monoclonal antibodies (MAbs) that had been previously raised against a human isolate of SV5. CPI- virus was
Junko Hasegawa-Minato et al.
Oncotarget, 9(3), 3483-3496 (2018-02-10)
c-Myc is a master regulator of various oncogenic functions in many types of human cancers. However, direct c-Myc-targeted therapy has not been successful in the clinic. Here, we explored a novel therapeutic target, which shows synthetic lethality in c-Myc-driven ovarian
Suena Ji et al.
Nature communications, 9(1), 3508-3508 (2018-08-31)
Regulated secretion is an essential process where molecules destined for export are directed to membranous secretory granules, where they undergo packaging and maturation. Here, we identify a gene (pgant9) that influences the structure and shape of secretory granules within the
S M Thomas et al.
Cell, 54(6), 891-902 (1988-09-09)
The "P" gene of the paramyxovirus SV5 encodes two known proteins, P (Mr approximately equal to 44,000) and V (Mr approximately equal to 24,000). The complete nucleotide sequence of the "P" gene has been obtained and is found to contain

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